Document Detail

Cell cycle progression in irradiated endothelial cells cultured from bovine aorta.
MedLine Citation:
PMID:  3186944     Owner:  NLM     Status:  MEDLINE    
Logarithmically growing endothelial cells from bovine aortas were exposed to single doses of 0-10 Gy of 60Co gamma rays, and cell cycle phase distribution and progression were examined by flow cytometry and autoradiography. In some experiments, cells were synchronized in the cell cycle with hydroxyurea (1 mM). Cell number in sham-irradiated control cultures doubled in approximately 24 h. Estimated cycle stage times for control cells were 14.4 h for G1 phase, 7.2 h for S phase, and 2.4 h for G2 + M phase. Irradiated cells demonstrated a reduced distribution at the G1/S phase border at 4 h, and an increased distribution in G2 + M phase at 24 h postirradiation. Autoradiographs of irradiated cells after continuous [3H]thymidine labeling indicated a block in G1 phase or at the G1/S-phase border. The duration of the block was dose dependent (2-3 min/cGy). Progression of the endothelial cells through S phase after removal of the hydroxyurea block also was retarded by irradiation, as demonstrated by increased distribution in early S phase and decreased distribution in late S phase. These results indicate that progression of asynchronous cultured bovine aortic endothelial cells through the DNA synthetic cycle is susceptible to radiation inhibition at specific sites in the cycle, resulting in redistribution and partial synchronization of the population. Thus aortic endothelial cells, diploid cells from a normal tissue, resemble many immortal cell types that have been examined in this regard in vitro.
D B Rubin; E A Drab; W F Ward; K D Bauer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Radiation research     Volume:  116     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-22     Completed Date:  1988-12-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  364-71     Citation Subset:  IM; S    
Department of Medicine, Rush-Presbyterian/St. Luke's Medical Center, Chicago, Illinois 60612.
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MeSH Terms
Aorta / cytology,  radiation effects*
Cell Cycle / radiation effects*
Cobalt Radioisotopes
Endothelium, Vascular / cytology,  radiation effects*
Gamma Rays
Grant Support
Reg. No./Substance:
0/Cobalt Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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