Document Detail

Cell cycle phase specificity of hydroxyurea and its effects on the cell kinetics in embryonic spinal cord.
MedLine Citation:
PMID:  7385059     Owner:  NLM     Status:  MEDLINE    
Pregnant mice (NMRI) received an intravenous injection of 500 mg/kg of hydroxyurea (HU) on day 10 of gestation. The animals were killed 1, 1.5, 2, 2.5, 4, 6, 8 or 10 h after application of the drug and the embryos removed. Forty-five min. prior to sacrifice, the animals were given an intraperitoneal injection of 5 microCi/g 3H-thymidine (specific activity 5 Ci/mmol). From autoradiographs of the embryos the percentage of mitoses, necroses and labelled cells of the spinal cord was determined at the various time intervals. It was observed that HU inhibited DNA-synthesis in all S-phase cells for more than 4 hours. All cells damaged by HU did not start DNA-synthesis again and became necrotic. Only cells entering the S-phase after the influence of HU could replicate their DNA in a normal manner. It is assumed that HU possibly blocks cells in the G2-phase and in the mitosis for a certain period of time.
R Herken
Related Documents :
19587119 - Divergent s phase checkpoint activation arising from prereplicative complex deficiency ...
9749709 - The rhythmicity of cells of the medial septum/diagonal band of broca in the awake freel...
17173999 - The effects of growth temperature and growth phase on the inactivation of listeria mono...
1676229 - On circadian rhythms in human epidermal cell proliferation.
17295519 - Aldo-keto reductase- and cytochrome p450-dependent formation of benzo[a]pyrene-derived ...
976389 - The influence of steroids on erythropoiesis in mouse fetal liver cell cultures: relevan...
11590319 - Expression of p57/kip2 protein in pancreatic adenocarcinoma.
23389739 - Comparison of toxicity between the different-type tio(2) nanowires in vivo and in vitro.
2485189 - Study of h-tsh immunoreactive adenohypophyseal cells following treatment with methymazole.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Teratology     Volume:  21     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-08-25     Completed Date:  1980-08-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9-14     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / drug effects*
DNA Replication / drug effects
Gestational Age
Hydroxyurea / adverse effects*
Interphase / drug effects
Mice / embryology*
Mitosis / drug effects
Spinal Cord / cytology*,  embryology
Time Factors
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Syndromes of cardiovascular malformations induced by copper citrate in hamsters.
Next Document:  Pulmonary endothelial pavement patterns.