Document Detail


Cell cycle phase specificity of hydroxyurea and its effects on the cell kinetics in embryonic spinal cord.
MedLine Citation:
PMID:  7385059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnant mice (NMRI) received an intravenous injection of 500 mg/kg of hydroxyurea (HU) on day 10 of gestation. The animals were killed 1, 1.5, 2, 2.5, 4, 6, 8 or 10 h after application of the drug and the embryos removed. Forty-five min. prior to sacrifice, the animals were given an intraperitoneal injection of 5 microCi/g 3H-thymidine (specific activity 5 Ci/mmol). From autoradiographs of the embryos the percentage of mitoses, necroses and labelled cells of the spinal cord was determined at the various time intervals. It was observed that HU inhibited DNA-synthesis in all S-phase cells for more than 4 hours. All cells damaged by HU did not start DNA-synthesis again and became necrotic. Only cells entering the S-phase after the influence of HU could replicate their DNA in a normal manner. It is assumed that HU possibly blocks cells in the G2-phase and in the mitosis for a certain period of time.
Authors:
R Herken
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Teratology     Volume:  21     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-08-25     Completed Date:  1980-08-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / drug effects*
DNA Replication / drug effects
Female
Gestational Age
Hydroxyurea / adverse effects*
Interphase / drug effects
Kinetics
Mice / embryology*
Mitosis / drug effects
Necrosis
Pregnancy
Spinal Cord / cytology*,  embryology
Time Factors
Chemical
Reg. No./Substance:
127-07-1/Hydroxyurea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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