| Cell cycle phase-dependent changes of localization and oligomerization states of nucleophosmin / B23. | |
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MedLine Citation:
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PMID: 8526928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleophosmin / B23, an abundant nucleolar phosphoprotein, accumulates in the nucleoplasm of cells during the stationary phase of growth or after exposure to selected cytotoxic drugs [Chan, P.K. (1992) Exp. Cell Res. 203, 174-181]. Monomeric and hexameric forms of nucleophosmin / B23 are present in cells [Yung, B.Y.M. and Chan, P.K. (1987) Biochim. Biophys. Acta. 925, 74-82]. Using indirect immunofluorescence, here we show that there are changes in nucleophosmin / B23's cellular localizations throughout the cell cycle. The alternation of the nuclear and nucleolar localizations of nucleophosmin / B23 is most frequently observed in cells of G1 and G1/S phases. The incidence of the changes of localizations of nucleophosmin / B23 decreases as cells enter into S and G2 phases. In parallel, using Western blotting, the reversible change of oligomerization states between the hexameric and monomeric forms of nucleophosmin / B23 is also found to occur most frequently in cells of G1 and G1/S phases. As cells progressed into S, G2 and M phases, the frequency of the reversible change of hexameric and monomeric forms of nucleophosmin / B23 decreases. These findings suggest that nucleophosmin / B23 being possibly involved in rRNA processing and transport, is highly active at G1 and G1/S phases as demonstrated by the dynamic, reversible changes of localization and oligomerization states of nucleophosmin / B23. |
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Authors:
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Y H Chou; B Y Yung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 217 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1995 Dec |
Date Detail:
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Created Date: 1996-01-19 Completed Date: 1996-01-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 313-25 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Chang Gung Medical & Engineering College, Tao-Yuan, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport, Active Cell Cycle / physiology* Cell Nucleolus / metabolism Cell Nucleus / metabolism Fluorescent Antibody Technique, Indirect Hela Cells Humans Nuclear Proteins / chemistry, metabolism* Phosphoproteins / chemistry, metabolism* Protein Conformation RNA Processing, Post-Transcriptional RNA, Ribosomal / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Proteins; 0/Phosphoproteins; 0/RNA, Ribosomal; 117896-08-9/nucleophosmin |
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