Document Detail


Cell cycle phase, cellular Ca2+ and development in Dictyostelium discoideum.
MedLine Citation:
PMID:  11330860     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Dictyostelium discoideum, the initial differentiation of cells is regulated by the phase of the cell cycle at starvation. Cells in S and early G2 (or with a low DNA content) have relatively high levels of cellular Ca2+ and display a prestalk tendency after starvation, whereas cells in mid to late G2 (or with a high DNA content) have relatively low levels of Ca2+ and display a prespore tendency. We found that there is a correlation between cytosolic Ca2+ and cell cycle phase, with high Ca2+ levels being restricted to cells in the S and early G2 phases. As expected on the basis of this correlation, cell cycle inhibitors influence the proportions of amoebae containing high or low Ca2+. However, it has been reported that in the rtoA mutant, which upon differentiation gives rise to many more stalk cells than spores (compared to the wild type), initial cell-type choice is independent of cell cycle phase at starvation. In contrast to the wild type, a disproportionately large fraction of rtoA amoebae fall into the high Ca2+ class, possibly due to an altered ability of this mutant to transport Ca2+.
Authors:
M Azhar; P K Kennady; G Pande; M Espiritu; W Holloman; D Brazill; R H Gomer; V Nanjundiah
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  45     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  405-14     Citation Subset:  IM    
Affiliation:
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Cell Aggregation
Cell Cycle Proteins / metabolism*
Cell Differentiation / physiology*
DNA, Protozoan / analysis
Dictyostelium / cytology*,  physiology
Flow Cytometry
G2 Phase / physiology
Protozoan Proteins*
S Phase / physiology
Spores
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA, Protozoan; 0/Protozoan Proteins; 0/RtoA protein, Dictyostelium discoideum; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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