Document Detail


Cell cycle perturbations following DNA damage in the presence of ADP-ribosylation inhibitors.
MedLine Citation:
PMID:  2988807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell cycle analysis by DNA flow cytofluorimetry and autoradiography has been utilized to investigate the effects of 3-methoxybenzamide (MBA), a potent inhibitor of ADP-ribosylation reactions, on cell cycle progression in N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-treated C3H10T1/2 cells. Following a dose of 6.8 microM MNNG, the presence of MBA resulted in an increased length of S phase from approximately 6.5 h to 10 h and in an accumulation of cells in G2 with a mitosis delay of 12 h. Progression to the next S phase occurred 5-10 times more slowly and the cells ultimately accumulated in G2. Increasing the dose of MNNG resulted in a complete block in cell division in the absence of ADP-ribosylation. These results suggest that ADP-ribosylation reactions, which do not seem to be necessary for DNA excision repair in nondividing cells, are essential for coordinating the events of DNA excision repair with DNA replication and events related to progression through the cell cycle.
Authors:
E L Jacobson; R Meadows; J Measel
Related Documents :
1167367 - Synthesis of glycolipids and phospholipids in hamster cells: dependence on cell density...
3324807 - Cell cycle phase-specific perturbation of hepatic tumor cell growth kinetics during sho...
7579177 - Cell cycle regulation during growth-dormancy cycles in pea axillary buds.
3186937 - Cell progression after selective irradiation of dna during the cell cycle.
2328267 - Dielectrophoretic characterisation of friend murine erythroleukaemic cells as a measure...
2455377 - Intermediate filament expression in normal parotid glands and pleomorphic adenomas.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  6     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-08-01     Completed Date:  1985-08-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  711-4     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Benzamides / toxicity*
Cell Cycle / drug effects
Cells, Cultured
DNA Repair / drug effects*
Methylnitronitrosoguanidine
Mice
NAD+ Nucleosidase / antagonists & inhibitors*
Poly Adenosine Diphosphate Ribose / physiology
Poly(ADP-ribose) Polymerases / antagonists & inhibitors*
Grant Support
ID/Acronym/Agency:
CA23994/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Benzamides; 26656-46-2/Poly Adenosine Diphosphate Ribose; 5813-86-5/3-methoxybenzamide; 70-25-7/Methylnitronitrosoguanidine; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.2.2.5/NAD+ Nucleosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhalation studies on the effects of tremolite and brucite dust in rats.
Next Document:  Cellular recovery of dividing and confluent C3H10T1/2 cells from N-methyl-N'-nitro-N-nitrosoguanidin...