Document Detail


Cell cycle perturbation in a human hepatoblastoma cell line constitutively expressing Hepatitis C virus core protein.
MedLine Citation:
PMID:  14689276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hepatitis C virus (HCV) is one of the major causes of chronic liver disease with the potential for development of hepatocellular carcinoma (HCC). The core protein of HCV has been shown to modulate expression of various cellular genes and to influence a number of cellular functions. We investigated the effect of constitutively expressed HCV core protein on cell cycle progression in HepG2 cell line, which is derived from a differentiated human hepatoblastoma and shows biosynthetic features similar to human hepatocytes. The results indicated that stable expression of the core protein in unsynchronized HepG2 cells induced a perturbation of the cell cycle with reduced cell doubling meantime and increased S phase fraction. Increase of c-myc protein above the basal expression level was demonstrated with a significant increase of c-myc stability, as revealed by its prolonged intracellular half-life, in HepG2 expressing HCV core protein. In contrast, p53 and p21 levels were unchanged. These results suggest that HCV core protein may promote cell cycle progression in HepG2 cells possibly through increasing stability of c-myc oncoprotein. These results are in support of important role played by HCV core protein in virus-mediated pathogenesis in persistently infected hosts and in hepatocarcinogenesis.
Authors:
A Ruggieri; M Murdolo; T Harada; T Miyamura; M Rapicetta
Related Documents :
11319606 - Staurosporine-induced apoptosis of hpv positive and negative human cervical cancer cell...
1651476 - Anti-sense phosphorothioate oligonucleotides have both specific and non-specific effect...
14704126 - Wild type p53 increased chemosensitivity of drug-resistant human hepatocellular carcino...
15077166 - Cyr61 suppresses the growth of non-small-cell lung cancer cells via the beta-catenin-c-...
21851146 - Genotoxic and cytotoxic activity of host defense peptides against human soft tissue sar...
15259856 - Influence of a novel platinum compound--cis-dichloro (dimethylsulphoxide) (1-beta-d-ryb...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-09-22
Journal Detail:
Title:  Archives of virology     Volume:  149     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-22     Completed Date:  2004-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  61-74     Citation Subset:  IM    
Affiliation:
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Cell Division
Cell Line, Tumor
Hepatoblastoma / metabolism,  pathology,  virology*
Humans
Liver Neoplasms / metabolism,  pathology,  virology*
Proto-Oncogene Proteins c-myc / metabolism
Viral Core Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-myc; 0/Viral Core Proteins; 0/nucleocapsid protein, Hepatitis C virus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  High efficiency gene transfer into mammalian kidney cells using baculovirus vectors.
Next Document:  Fucosylated lactosamines participate in adhesion of HIV-1 envelope glycoprotein to dendritic cells.