| Cell cycle, morphology and pluripotency of octaploid embryonic stem cells in comparison with those of tetraploid and diploid cells. | |
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MedLine Citation:
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PMID: 19624307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To examine the alteration of cellular characteristics on ploidy transition of embryonic stem (ES) cells, octaploid cells (8H1 cells) were established from tetraploid H-1 (ES) cells, and compared with tetraploid and diploid H-1 (ES) cells (4H1 and 2H1 cells, respectively). The duration of G(1), S, and G(2)/M phases were essentially the same among 2H1, 4H1, and 8H1 cells, suggesting that cell cycle progression is conserved. The ratio of cell volume of 2H1, 4H1, and 8H1 cells was about 1 : 2 : 4, indicating that these polyploid cells were generated through cell cycle progression without cell division. The morphology of 8H1 cells was flagstone-like and flatter than that of 4H1 cells, and differed from the spindle-like shape of 2H1 cells, suggesting that transformation occurred during the ploidy transitions. Alkaline phosphatase activity was expressed equivalently in 2H1, 4H1, and 8H1 cells, and solid tumors that contained endodermal, mesodermal, and ectodermal cells were formed by 2H1, 4H1 or 8H1 cells after interperitoneal injection into the mouse abdomen, suggesting that pluripotency was preserved in the ploidy transition. |
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Authors:
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Kohzaburo Fujikawa-Yamamoto; Minoru Miyagoshi; Hiroko Yamagishi |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2009-06-23 |
Journal Detail:
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Title: Human cell : official journal of Human Cell Research Society Volume: 22 ISSN: 1749-0774 ISO Abbreviation: Hum. Cell Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-23 Completed Date: 2009-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912329 Medline TA: Hum Cell Country: Japan |
Other Details:
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Languages: eng Pagination: 64-71 Citation Subset: IM |
Affiliation:
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Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, Japan. fujikawa@kanazawa-med.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Cell Culture Techniques Cell Cycle* Cells, Cultured Embryonic Stem Cells / cytology*, enzymology, pathology Mice Mice, Inbred C3H Mice, Inbred Strains Pluripotent Stem Cells / cytology*, enzymology, pathology Polyploidy* Stem Cell Transplantation Teratocarcinoma / pathology |
| Chemical | |
Reg. No./Substance:
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EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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