Document Detail

Cell cycle, melanin contents and apoptosis processes in B16 and Cloudman S91 mouse melanoma cells after exposure to cytostatic drugs.
MedLine Citation:
PMID:  18540169     Owner:  NLM     Status:  MEDLINE    
Free radicals are generated in cells during many metabolic processes and eliminated from an organism by complex enzymatic and nonenzymatic systems. Many chemical compounds--among others melanin, reveal antioxidative properties. In this work the influence of some cytostatic drugs (at EC50) on melanin content and on the apoptotic processes in mouse melanoma B16 and Cloudman S91 cells in vitro were investigated. The cells were incubated with adriblastin, actinomycin D, cytosine arabinoside, cisplatin, dacarbazine and vincristine. The number of viable mouse melanoma B16 and Cloudman S91 cells was estimated by flow cytometry analysis and melanin content in colorimetric assays. Apoptotic cells were detected using the annexin V-FITC test. The majority of tested cytostatic drugs caused an increase of melanin content in the cells of both melanoma lines, except cisplatin and dacarbazine in the case of B16 cells and dacarbazine in the case of Cloudman S91. Adriblastin, actinomycin D and vincristine evoked apoptosis in the both tested cell lines. Slight increase of melanin content in melanoma cells can be a cell answer to free radicals generation by some cytostatic drugs like adriblastin, actinomycin D and vincristine.
Dorota M Olszewska-Słonina; Jan Styczyński; Rafał Czajkowski; Tomasz A Drewa; Dariusz Musiałkiewicz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta poloniae pharmaceutica     Volume:  64     ISSN:  0001-6837     ISO Abbreviation:  Acta Pol Pharm     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2008-06-10     Completed Date:  2008-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985167R     Medline TA:  Acta Pol Pharm     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  469-78     Citation Subset:  IM    
Department of Medical Biology, Collegium Medicum, Nicolaus Copernicus University, Karłowicza 24, PL 85-092 Bydgoszcz, Poland.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Cell Cycle / drug effects
Cell Line, Tumor
Cisplatin / pharmacology
Cytarabine / pharmacology
Dactinomycin / pharmacology
Melanins / analysis*
Melanoma, Experimental / chemistry,  drug therapy*,  pathology
Mice, Inbred C57BL
Vincristine / pharmacology
Reg. No./Substance:
0/Antineoplastic Agents; 0/Melanins; 147-94-4/Cytarabine; 15663-27-1/Cisplatin; 50-76-0/Dactinomycin; 57-22-7/Vincristine

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