| Cell cycle-mediated structural and functional alteration of P85gag-mos protein kinase activity. | |
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MedLine Citation:
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PMID: 2138725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated cell cycle-dependent regulation of protein kinase activity encoded by the viral mos gene by using a normal rat kidney cell line (NRK-6m2) chronically infected with a temperature-sensitive mutant (ts110) of Moloney murine sarcoma virus, which produces the P85gag-mos transforming protein. In elutriation experiments, in which cells in various phases of the cell cycle are separated based upon size, a twofold increase in the specific activity of the P85gag-mos protein kinase was observed as cells progressed from G0/G1 through S and G2/M. A three- to fourfold increase in gas-mos protein kinase specific activity relative to unsynchronized cells was observed in mitotic NRK-6m2 cells synchronized by treatment with thymidine followed by colcemid or with nocodazole alone. Interestingly, the gag-mos protein was structurally altered in mitotic cells generating a protein species moving slower than P85gag-mos in SDS-polyacrylamide gels. Our findings indicate that viral mos protein kinase activity is regulated during the cell cycle via phosphorylation. We propose that the mos transforming protein functions in a pleiotropic manner, affecting both cytoplasmic and nuclear targets. |
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Authors:
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J X Liu; B Singh; D Wlodek; R B Arlinghaus |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Oncogene Volume: 5 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1990 Feb |
Date Detail:
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Created Date: 1990-05-04 Completed Date: 1990-05-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 171-8 Citation Subset: IM |
Affiliation:
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Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Cell Cycle Cell Separation Immunoblotting Mitosis Oncogene Proteins v-mos Peptide Mapping Phosphorylation Protein-Tyrosine Kinases / analysis* Rats Retroviridae Proteins, Oncogenic / analysis*, immunology, physiology |
| Grant Support | |
ID/Acronym/Agency:
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CA16672/CA/NCI NIH HHS; CA45125/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Oncogene Proteins v-mos; 0/Retroviridae Proteins, Oncogenic; EC 2.7.10.1/Protein-Tyrosine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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