| Cell cycle kinetic effects of tamoxifen on human breast cancer cells. Flow cytometric analyses of DNA content, BrdU labeling, Ki-67, PCNA, and statin expression. | |
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MedLine Citation:
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PMID: 7904138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tamoxifen is known to inhibit the growth of some human mammary carcinoma cells; this effect is accompanied by a decrease in the proportion of cells synthesizing DNA. In this work, flow cytometry of DNA and of bromodeoxyuridine labeling and the evaluation of the cell cycle-related antigens Ki-67, PCNA, and statin were used to investigate the changes in the proliferation kinetics of MCF-7 cells before and after treatment with 10(-7) M TAM. The treatment with TAM induced a significant decrease in the fraction of S-phase cells and an increase in those with a DNA content typical of G0/1 phase. The TAM-induced block in G0/1 is paralleled by a decrease in the frequency of cells expressing Ki-67 and PCNA, and by an increase in statin-positive (G0) cells. These results confirmed that the TAM-induced inhibition of cell growth is associated with major changes in the cell cycle parameters of MCF-7 cells, and provide the first experimental evidence that two main mechanisms are operating: the accumulation of cells in G1, before the onset of S-phase, and the exit of some cells from the cycling compartment. |
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Authors:
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M Danova; C Pellicciari; C Zibera; R Mangiarotti; N Gibelli; M Giordano; E Wang; G Mazzini; A Riccardi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 698 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1994-02-04 Completed Date: 1994-02-04 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 174-81 Citation Subset: IM |
Affiliation:
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Dipartimento di Medicina Interna e Terapia Medica, I.R.C.C.S. Policlinico San Matteo, University of Pavia, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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pathology* Bromodeoxyuridine Cell Cycle / drug effects* Cell Cycle Proteins Cell Line DNA, Neoplasm / analysis, metabolism* Female Flow Cytometry / methods Fluorescent Antibody Technique Humans Immunohistochemistry / methods Ki-67 Antigen Kinetics Neoplasm Proteins / analysis*, biosynthesis Nuclear Proteins / analysis*, biosynthesis Proliferating Cell Nuclear Antigen Protein Biosynthesis Proteins / analysis* Tamoxifen / toxicity* Tumor Cells, Cultured Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA, Neoplasm; 0/EEF1A2 protein, human; 0/Ki-67 Antigen; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/Proliferating Cell Nuclear Antigen; 0/Proteins; 0/Tumor Markers, Biological; 10540-29-1/Tamoxifen; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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