| Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold. | |
| | |
MedLine Citation:
|
PMID: 9096339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cell cycle regulating Cdc2 protein kinase helps orchestrate cell cycle dependent changes in cell structure and function. This report shows that Cdc2 is localized to the centrosome region and is tightly bound to the nuclear matrix-intermediate filament scaffold. Antibodies to Cdc2 and to the centrosome-specific protein, pericentrin, label the centrosome in an apparently cell cycle independent manner. Isolated centrosomes also label similarly with both antibodies. Essentially, all cells show Cdc2 labeling of the centrosomes, implying an independence of the stage in the cell cycle, a conclusion supported by studies of synchronized cells. In contrast to the labeling of every cell with the Cdc2 monoclonal antibody, fewer centrosomes were labeled with an antibody to the PSTAIRE domain of Cdc2. Embedment-free, immunogold electron micrographs of extracted cell whole mounts show the centrioles and a pericentriolar network of filaments. Both Cdc2 and pericentrin antibodies decorate the amorphous pericentriolar material, while the Cdc2 antibodies also decorate the centrioles themselves. The constitutive presence of Cdc2 at the centrosome suggests a continuing role in the dynamics of centrosome function throughout the cell cycle. |
| | |
Authors:
|
S M Pockwinse; G Krockmalnic; S J Doxsey; J Nickerson; J B Lian; A J van Wijnen; J L Stein; G S Stein; S Penman |
Related Documents
:
|
15994309 - Depletion of anaphase-promoting complex or cyclosome (apc/c) subunit homolog apc1 or cd... 20975879 - Low-grade spindle-cell ameloblastic carcinoma: report of an unusual case with immunohis... 12628189 - Exit from exit: resetting the cell cycle through amn1 inhibition of g protein signaling. 18628309 - Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells. 17606999 - Centrosome replication in hydroxyurea-arrested cho cells expressing gfp-tagged centrin2. 19171759 - Actin filament dynamics are dominated by rapid growth and severing activity in the arab... 15789219 - Ontogeny of estrogen receptor (er) alpha and its co-localization with pituitary hormone... 8365569 - Crumbs and stardust act in a genetic pathway that controls the organization of epitheli... 1965839 - Characterization of functional nerve growth factor-receptors in a cns glial cell line: ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 94 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1997 Apr |
Date Detail:
|
Created Date: 1997-05-08 Completed Date: 1997-05-08 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3022-7 Citation Subset: IM |
Affiliation:
|
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
CDC2 Protein Kinase
/
metabolism* Cell Cycle* Cell Line Centrosome / metabolism* Cytoskeleton / ultrastructure Fluorescent Antibody Technique Hela Cells Humans Intermediate Filaments / metabolism* Microscopy, Electron Nuclear Matrix / metabolism* Protein Binding |
| Grant Support | |
ID/Acronym/Agency:
|
AR42262/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 2.7.11.22/CDC2 Protein Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of a novel pathway important for proliferation and differentiation of primary erythro...
Next Document: Ras-independent transformation by v-Src.