Document Detail

Cell cycle features of primate embryonic stem cells.
MedLine Citation:
PMID:  16239321     Owner:  NLM     Status:  MEDLINE    
Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G1 after gamma-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G1 phase are reduced or absent.
Anne-Catherine Fluckiger; Guillaume Marcy; Mélanie Marchand; Didier Négre; François-Loïc Cosset; Shoukhrat Mitalipov; Don Wolf; Pierre Savatier; Colette Dehay
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-20
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  24     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-04-03     Completed Date:  2006-06-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  547-56     Citation Subset:  IM    
INSERM, U371, Cerveau et Vision, Department of Stem Cells and Cortical Development, 18 avenue Doyen Lépine, 69500 Bron, France.
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MeSH Terms
Cells, Cultured
Cyclin E / biosynthesis*
Embryo, Mammalian / cytology*,  metabolism
G1 Phase / physiology,  radiation effects
Gamma Rays
Gene Expression Regulation / radiation effects
Macaca mulatta
Signal Transduction / physiology,  radiation effects
Species Specificity
Stem Cells / cytology*,  metabolism
Reg. No./Substance:
0/Cyclin E

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