| Cell cycle events mediate lactacystin-induced apoptotic death of neuronal PC12 cells. | |
| | |
MedLine Citation:
|
PMID: 20658965 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Dysfunction of the UPS (ubiquitin-proteasome system) has been implicated in dopaminergic neuronal death in PD (Parkinson's disease). Recent studies suggest that unregulated cell cycle events play a key role in neuronal death. In this study, the effects of UPS dysfunction on cell cycle events in neuronal differentiated PC12 cells were analysed using a specific inhibitor of proteasome, lactacystin. Lactacystin induced apoptosis, G₂/M cell cycle arrest and sustained the phosphorylation of the pRB (retinoblastoma protein), the key molecular process of G₁/S transition, in neuronal PC12 cells. Furthermore, inhibition of cell cycle progression protected against lactacystin-induced cell apoptosis. Finally, we determined that lactacystin activated the ERK signalling pathway. Inhibition of ERK1/2 activation by MEK-1 inhibitor PD98059 decreased cell cycle aberrant and prevented apoptosis induced by lactacystin. These results indicate that aberrant cell cycle events contribute to apoptotic death induced by UPS dysfunction. |
| | |
Authors:
|
Zhentao Zhang; Yan Xu; Kairong Qin; Tao Wang; Xuebing Cao |
Related Documents
:
|
10362255 - Instabilities in phosphorylation-dephosphorylation cascades and cell cycle checkpoints. 8573995 - The interchromatin granules. 15655375 - Characterization and gene expression profiling of a stable cell line expressing a cell ... 15371535 - A nima-related kinase, fa2p, localizes to a novel site in the proximal cilia of chlamyd... 8404365 - Increased membrane permeability of apoptotic thymocytes: a flow cytometric study. 6300455 - Detection of an 85,000-dalton phosphoprotein in ts110 murine sarcoma virus-infected cel... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell biology international Volume: 34 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 1181-7 Citation Subset: IM |
Affiliation:
|
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Peoples Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of an ATP regulated DNA processing enzyme and thermotolerant phosphoesterase in a r...
Next Document: Interchromatin granule clusters of the scorpionfly oocytes contain poly(A)+ RNA, heterogeneous ribon...