| Cell cycle and drug sensitivity. | |
| | |
MedLine Citation:
|
PMID: 15911969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Induction of tumor cell death by chemotherapeutic modalities often occurs in a cell cycle-dependent manner. It has also been observed that several regulatory proteins involved in tumor chemosensitivity and apoptosis are expressed periodically during the cell cycle progression. However, the nature of cancer cellular chemosensitivity and mechanisms of action of anticancer drugs in different phases of the cell cycle still remain unknown. In this chapter we describe two methods (serum deprivation and pharmacological drug treatment) to synchronize human tumor cells in specific phases of the cell cycle, followed by induction of cell death by anticancer drugs. We also describe three methods (flow cytometry, Western blot, and DNA fragmentation) to measure the cell cycle-associated cytotoxic effects of chemotherapeutic agents. |
| | |
Authors:
|
Aslamuzzaman Kazi; Q Ping Dou |
Related Documents
:
|
9451419 - Wr-1065, an active metabolite of the cytoprotector amifostine, affects phosphorylation ... 12840909 - Multiple defects of cell cycle checkpoints in u937-aspi3k, an u937 cell mutant stably e... 12072179 - Investigating the relationship between the cell cycle and apoptosis using flow cytometry. 16662459 - Interaction of the circadian cycle with the cell cycle in pyrocystis fusiformis. 4093469 - An investigation of the centriole cycle using 3t3 and cho cells. 3325079 - Cell cycle controls and the role of nerves and the regenerate epithelium in urodele for... 10576149 - Transforming growth factor-beta1 protein, proliferation and apoptosis of oval cells in ... 10944199 - Contact with fibrillar collagen inhibits melanoma cell proliferation by up-regulating p... 9049849 - The cell cycle and induction of apoptosis in a hamster fibrosarcoma cell line treated w... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Methods in molecular medicine Volume: 111 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 2005 |
Date Detail:
|
Created Date: 2005-05-24 Completed Date: 2005-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
|
Languages: eng Pagination: 33-42 Citation Subset: IM |
Affiliation:
|
Moffitt Cancer Center & Research Institute, University of South Florida, Tampa, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents
/
pharmacology* Apoptosis Blotting, Western Cell Cycle* / drug effects Culture Media, Serum-Free / pharmacology DNA / metabolism DNA Fragmentation Drug Screening Assays, Antitumor / methods* Flow Cytometry G1 Phase Humans |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Culture Media, Serum-Free; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro micronucleus technique to predict chemosensitivity.
Next Document: TUNEL assay as a measure of chemotherapy-induced apoptosis.