| Cell-cycle deregulation induced by three different root canal sealers in vitro. | |
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MedLine Citation:
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PMID: 18718791 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the effects of 3 root canal sealers on the cell cycle of mouse fibroblasts (3T3 cell line). Freshly mixed root canal sealers (AH Plus, Endofill, and Sealer 26) were eluted for 24 h using cell culture medium. Antiproliferation activity of different eluate dilutions was determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Cell cycle was subsequently evaluated by flow cytometry. Differences were tested by analysis of variance with Tukey test (P < .05). Results showed that the root canal sealers inhibited cell growth in a dose-dependent manner. Antiproliferation activity of the root canal sealers was associated with cell-cycle deregulation. This event involved cell-cycle arrest in the S/G2/M phases and an increase of the sub-G1 population, which are consistent with both cytostatic and cytotoxic effects. This information may contribute to a better understanding of the toxicity mechanism displayed by root canal sealers currently used in clinical practice. |
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Authors:
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Caroline R A Valois; Ricardo B Azevedo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-20 |
Journal Detail:
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Title: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics Volume: 106 ISSN: 1528-395X ISO Abbreviation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2009-02-02 Revised Date: 2012-03-05 |
Medline Journal Info:
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Nlm Unique ID: 9508562 Medline TA: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Country: United States |
Other Details:
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Languages: eng Pagination: 763-7 Citation Subset: D; IM |
Affiliation:
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Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília, Brazil. cravalois@uol.com.br |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Bismuth / toxicity Calcium Hydroxide / toxicity Cell Cycle / drug effects* Cell Proliferation / drug effects Dose-Response Relationship, Drug Epoxy Resins / toxicity Fibroblasts / drug effects* Flow Cytometry Materials Testing Mice Root Canal Filling Materials / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Epoxy Resins; 0/Root Canal Filling Materials; 0/Sealer 26; 0/expoy resin-based root canal sealer; 115055-53-3/Endo-Fill; 1305-62-0/Calcium Hydroxide; 7440-69-9/Bismuth |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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