Document Detail

Cell cycle-dependent vimentin expression in elutriator-synchronized, TPA-treated MPC-11 mouse plasmacytoma cells.
MedLine Citation:
PMID:  1563479     Owner:  NLM     Status:  MEDLINE    
We correlated cell cycle progression and vimentin expression at the single cell level by multiparameter flow cytometry in populations of MPC-11 cells enriched in different cell cycle phases by centrifugal elutriation and subsequently treated with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Synchronized, untreated cultures showed a uniform, synchronous progression through the cell cycle during further cultivation. A 6-h TPA treatment of G1-phase-enriched cultures induced both a partial G1-phase arrest in the same cycle and a moderate fraction of cells to become vimentin positive. However, nearly all cells of the cultures enriched in S- or in G2/M-phase cells could be arrested by TPA treatment at the earliest in the G1 phase of the second cell cycle and displayed higher fractions of positive cells as well as higher average levels of vimentin. After 20 h of treatment, the G1-phase arrest was almost complete. In terms of fractions of vimentin-positive cells as well as of average cellular vimentin content, the differences between the cultures resembled, albeit on a higher level, those between the respective cultures treated with TPA for 6 h. These observations might explain the striking bimodal distribution of individual cellular vimentin content detectable in G1-phase fractions of asynchronous, TPA-treated cultures. The pattern of vimentin mRNA accumulation in synchronized cultures after short-term TPA treatment strongly suggests that the cell cycle-dependent pattern of vimentin expression is caused, at least in part, by different levels of vimentin mRNA accumulated in the cells. Since proteinaceous mediator(s) are obviously involved in TPA-induced vimentin expression in MPC-11 cells, cell cycle-dependent vimentin expression in these cells may be dependent on cell cycle-dependent regulation of the activity and/or concentration of such mediator(s).
G Giese; M Kubbies; P Traub
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  200     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-18     Completed Date:  1992-05-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  118-25     Citation Subset:  IM    
Max-Planck-Institut für Zellbiologie, Rosenhof, Ladenburg, Germany.
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MeSH Terms
Cell Cycle / drug effects,  genetics*
Cell Separation
DNA / analysis
Flow Cytometry
Gene Expression / genetics*
Phorbol Esters / pharmacology*
Tumor Cells, Cultured / drug effects
Vimentin / biosynthesis*,  genetics
Reg. No./Substance:
0/Phorbol Esters; 0/Vimentin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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