Document Detail

Cell-cycle dependent tyrosine phosphorylation on mortalin regulates its interaction with fibroblast growth factor-1.
MedLine Citation:
PMID:  11162655     Owner:  NLM     Status:  MEDLINE    
We previously reported that endogenously expressed, intracellularly localized fibroblast growth factor (FGF)-1 interacts with mortalin. Here we report that FGF-1 added to the culture medium of quiescent BALB/c3T3 cells is taken up by the cells and interacts with mortalin in the cells in a regulated manner. Although both the internalized FGF-1 and mortalin were present at high levels throughout the FGF-1-initiated cell cycle, their interaction became apparent only in late G1 phase. Interestingly, mortalin was preferentially tyrosine phosphorylated at the same time, and when its normally weak phosphorylation in early G1 phase was augmented by treating the cells with vanadate, a strong interaction between mortalin and FGF-1 was established. Conversely, when phosphorylated mortalin was treated with tyrosine phosphatase, its interaction with FGF-1 was abrogated. These results indicate that FGF-1 taken up by cells preferentially interacts with mortalin in late G1 phase of the cell cycle, and that tyrosine phosphorylation of mortalin regulates this interaction.
E Mizukoshi; M Suzuki; T Misono; A Loupatov; E Munekata; S C Kaul; R Wadhwa; T Imamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  280     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1203-9     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, 305-8566, Japan.
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MeSH Terms
3T3 Cells
Carrier Proteins
Cell Cycle
Cell Line
DNA / metabolism
Electrophoresis, Polyacrylamide Gel
Fibroblast Growth Factor 1 / metabolism*,  pharmacokinetics
HSP70 Heat-Shock Proteins / metabolism*
Mice, Inbred BALB C
Microscopy, Fluorescence
Precipitin Tests
Protein Binding
Time Factors
Tyrosine / metabolism*
Vanadates / pharmacology
Reg. No./Substance:
0/Carrier Proteins; 0/HSP70 Heat-Shock Proteins; 0/Hspa9a protein, mouse; 0/Vanadates; 0/mortalin; 104781-85-3/Fibroblast Growth Factor 1; 55520-40-6/Tyrosine; 9007-49-2/DNA

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