Document Detail

Cell-cycle dependent transformation competence in Dictyostelium discoideum.
MedLine Citation:
PMID:  3395345     Owner:  NLM     Status:  MEDLINE    
We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells.
W Nellen; U Saur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  154     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-18     Completed Date:  1988-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  54-9     Citation Subset:  IM    
Max-Planck-Institut für Biochemie, Martinsried, FRG.
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MeSH Terms
Cell Cycle*
Dictyostelium / cytology,  genetics*,  growth & development
Transformation, Genetic*

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