| Cell-cycle dependent transformation competence in Dictyostelium discoideum. | |
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MedLine Citation:
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PMID: 3395345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a modification of the transformation procedure for Dictyostelium which allows for a more exact estimate of transformation efficiency and the isolation of primary transformants. Investigations of transformation competence revealed a negative correlation to cell density and a distinct distribution during the cell-cycle. In synchronized cells, transformation efficiency is 2-3 fold higher during mitosis when compared to unsynchronized cells. |
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Authors:
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W Nellen; U Saur |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 154 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Jul |
Date Detail:
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Created Date: 1988-08-18 Completed Date: 1988-08-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 54-9 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für Biochemie, Martinsried, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle* Dictyostelium / cytology, genetics*, growth & development Kinetics Transformation, Genetic* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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