Document Detail

Cell-cycle-dependent three-dimensional redistribution of nuclear proteins, P 120, pKi-67, and SC 35 splicing factor, in the presence of the topoisomerase I inhibitor camptothecin.
MedLine Citation:
PMID:  14597418     Owner:  NLM     Status:  MEDLINE    
Topoisomerase I (Topo I) is mostly known for its role in DNA relaxation, which is required for duplication and transcription. Topo I acts as a protein kinase mainly directed to the mRNA splicing factor SC35. Camptothecin is one of the specific Topo I inhibitors and is effective on the two functions of the enzyme. In this study we demonstrated that treatment of KB cells with camptothecin for only 30 min induced the 3D reorganization and redistribution of three proteins involved in the nucleus machinery, P 120, pKi-67, and SC 35, and this occurred in a cell cycle-dependent manner. Our data were obtained from confocal microscopic studies after immunolabeling, 3D reconstruction, and measurement of the nuclear components volumes. In the presence of camptothecin, P 120, which occupied the nucleolar volume, lost its reticulation and pKi-67 was redistributed within the nucleoplasm and even into the cytoplasm. Finally, for SC 35 the fusion of its dots into bigger volumes was observed specifically during the G1 phase. Variations of volumes were also observed for the nucleolus and for the nucleus. These results pointed out that, depending on the cell cycle phase, Topo I functions were selective toward the three different proteins.
Emmanuel Elias; Nathalie Lalun; Marianne Lorenzato; Laurent Blache; Pavel Chelidze; Marie-Françoise O'Donohue; Dominique Ploton; Hélène Bobichon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  291     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-04     Completed Date:  2003-12-11     Revised Date:  2011-09-27    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  176-88     Citation Subset:  IM    
Unité Médian, UMR CNRS 6142, UFR Pharmacie, IFR 53, 1 rue du Maréchal Juin, F 51096 Reims cedex, France.
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MeSH Terms
Camptothecin / pharmacology*
Cell Compartmentation / drug effects*,  physiology
Cell Cycle / drug effects,  genetics
Cell Line, Tumor
Cell Nucleus / drug effects,  metabolism*,  ultrastructure
Culture Media, Serum-Free / pharmacology
Cytoplasm / drug effects,  enzymology
DNA Topoisomerases, Type I / metabolism
Enzyme Inhibitors / pharmacology*
G1 Phase / drug effects,  genetics
Ki-67 Antigen / genetics,  metabolism
Microscopy, Confocal
Nuclear Proteins / genetics,  metabolism*
Topoisomerase I Inhibitors*
p120 GTPase Activating Protein / genetics,  metabolism
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Enzyme Inhibitors; 0/Ki-67 Antigen; 0/Nuclear Proteins; 0/Ribonucleoproteins; 0/Topoisomerase I Inhibitors; 0/p120 GTPase Activating Protein; 147153-65-9/SRSF2 protein, human; 7689-03-4/Camptothecin; EC Topoisomerases, Type I

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