Document Detail


Cell-cycle-dependent regulation of cell motility and determination of the role of Rac1.
MedLine Citation:
PMID:  15093740     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three key parameters describing cell motility: speed, persistence time and rate of diffusion. All investigated cell lines demonstrated a lower cell displacement in the G2 phase than in the G1/S phases. This was caused by a decrease in speed and/or persistence time. The decrease in motility was accompanied by changes in morphology reflecting the larger volume of cells in G2 than in G1. Furthermore, L-cells and HeLa-cells appeared to be less adherent in the G2 phase. Transfection of L-cells with constitutively active Rac1 led to a general increase in the speed and rate of diffusion in G2 to levels comparable to those of control cells in G1. In contrast, transfection with dominant-negative Rac1 reduced cell speed and resulted in cellular displacements, which were identical in G1 and G2. These observations indicate that migration of cultured cells is regulated in a cell-cycle-dependent manner, and that an enhancement of Rac1 activity is sufficient for a delay of the reduced cell displacement otherwise seen in G2.
Authors:
P S Walmod; R Hartmann-Petersen; S Prag; E L Lepekhin; C Röpke; V Berezin; E Bock
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  295     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-19     Completed Date:  2004-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  407-20     Citation Subset:  IM    
Affiliation:
Protein Laboratory, Institute of Molecular Pathology, University of Copenhagen, Copenhagen, Denmark. psw@plab.ku.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Cycle*
Cell Division
Cell Line, Tumor
Cell Movement / physiology*
Cell Size
Flow Cytometry
G2 Phase
Glioma / pathology
Hela Cells
Humans
Image Processing, Computer-Assisted
Kinetics
L Cells (Cell Line)
Mice
Microscopy, Fluorescence
Rats
Transfection
Video Recording
rac1 GTP-Binding Protein / genetics,  physiology*
Chemical
Reg. No./Substance:
EC 3.6.5.2/rac1 GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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