Document Detail


Cell cycle-dependent phosphorylation of the retinoblastoma-related protein p130.
MedLine Citation:
PMID:  7651744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The retinoblastoma-related protein p130 is a putative negative regulator of cell proliferation in mammalian cells. In this study, p130 is shown to exist in multiple phosphorylated forms in human cells. In glioblastoma T98G cells synchronized by serum deprivation, specific phosphorylated forms of p130 are found at different times after serum re-stimulation. Two phosphorylated forms of p130 only found in serum-arrested T98G cells and in early G1 phase associate with the adenovirus oncoprotein E1A in vitro. One of these two forms corresponds to the in vivo E1A-associated p130 in 293 cells, which express endogenous E1A protein. Moreover, p130 undergoes an abrupt shift to a unique phosphorylated form in mid G1 which is the only p130 form found during the remaining phases of the cell cycle. This phosphorylated form possesses an associated histone H1 kinase activity that is most active in late S phase and G2/M. The cell cycle-dependent expression pattern of cyclins in T98G cells is compatible with cyclin D1/CDK complexes driving the shift to this phosphorylated p130 form in mid G1. These results suggest that the putative growth inhibitory function of p130 is regulated by phosphorylation of this protein. They also suggest that differential phosphorylation of p130 during the cell cycle plays distinct roles in the regulation of p130 function.
Authors:
X Mayol; J Garriga; X Graña
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  11     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-27     Completed Date:  1995-09-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  801-8     Citation Subset:  IM    
Affiliation:
Fels Institute for Cancer Research, Temple University School of Medicine, Philadelphia, PA 19140, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenovirus E1A Proteins / biosynthesis,  metabolism
Brain Neoplasms
Cell Cycle*
Cell Line
Cyclin-Dependent Kinases / metabolism
Cyclins / metabolism
G1 Phase
Glioblastoma
Humans
Kinetics
Phosphoproteins / metabolism*
Phosphorylation
Proteins*
Retinoblastoma Protein / metabolism*
Retinoblastoma-Like Protein p130
Time Factors
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Adenovirus E1A Proteins; 0/Cyclins; 0/Phosphoproteins; 0/Proteins; 0/RBL2 protein, human; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p130; EC 2.7.11.22/Cyclin-Dependent Kinases

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