| Cell cycle-dependent phosphorylation of the retinoblastoma-related protein p130. | |
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MedLine Citation:
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PMID: 7651744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The retinoblastoma-related protein p130 is a putative negative regulator of cell proliferation in mammalian cells. In this study, p130 is shown to exist in multiple phosphorylated forms in human cells. In glioblastoma T98G cells synchronized by serum deprivation, specific phosphorylated forms of p130 are found at different times after serum re-stimulation. Two phosphorylated forms of p130 only found in serum-arrested T98G cells and in early G1 phase associate with the adenovirus oncoprotein E1A in vitro. One of these two forms corresponds to the in vivo E1A-associated p130 in 293 cells, which express endogenous E1A protein. Moreover, p130 undergoes an abrupt shift to a unique phosphorylated form in mid G1 which is the only p130 form found during the remaining phases of the cell cycle. This phosphorylated form possesses an associated histone H1 kinase activity that is most active in late S phase and G2/M. The cell cycle-dependent expression pattern of cyclins in T98G cells is compatible with cyclin D1/CDK complexes driving the shift to this phosphorylated p130 form in mid G1. These results suggest that the putative growth inhibitory function of p130 is regulated by phosphorylation of this protein. They also suggest that differential phosphorylation of p130 during the cell cycle plays distinct roles in the regulation of p130 function. |
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Authors:
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X Mayol; J Garriga; X Graña |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 11 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1995-09-27 Completed Date: 1995-09-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 801-8 Citation Subset: IM |
Affiliation:
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Fels Institute for Cancer Research, Temple University School of Medicine, Philadelphia, PA 19140, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenovirus E1A Proteins
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biosynthesis,
metabolism Brain Neoplasms Cell Cycle* Cell Line Cyclin-Dependent Kinases / metabolism Cyclins / metabolism G1 Phase Glioblastoma Humans Kinetics Phosphoproteins / metabolism* Phosphorylation Proteins* Retinoblastoma Protein / metabolism* Retinoblastoma-Like Protein p130 Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Adenovirus E1A Proteins; 0/Cyclins; 0/Phosphoproteins; 0/Proteins; 0/RBL2 protein, human; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p130; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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