| Cell cycle dependent nucleolar localization of the coronavirus nucleocapsid protein. | |
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MedLine Citation:
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PMID: 17426449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nucleolus is a dynamic sub-nuclear structure which is involved in ribosome subunit biogenesis, modulation of cell growth and response to cell stress. The nucleolar proteome varies particularly with regard to the cell cycle. Viral proteins can localise to the nucleolus and using the coronavirus nucleocapsid (N) protein as a model, the cell cycle dependent trafficking of viral proteins to the nucleolus was investigated. Cell synchronisation studies coupled to live cell confocal microscopy indicated that nucleolar localisation of N protein was greater in the G2/M phase of the cell cycle than at other stages. This result was reinforced when FRAP and FLIP analysis indicated that N protein was more mobile within the nucleoplasm and nucleolus in the G2/M phase of the cell cycle. The data suggested that viral nucleolar proteins can also localise to the nucleolus in a cell cycle dependent manner and this may be related to dynamic trafficking. |
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Authors:
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Ryan Cawood; Sally M Harrison; Brian K Dove; Mark L Reed; Julian A Hiscox |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 6 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-11 Completed Date: 2007-05-09 Revised Date: 2007-06-28 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 863-7 Citation Subset: IM |
Affiliation:
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Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus
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physiology Animals Cell Compartmentation / physiology Cell Cycle / genetics* Cell Nucleolus / genetics, metabolism* Cercopithecus aethiops Coronavirus / genetics, metabolism* Coronavirus Infections / genetics, metabolism Fluorescence Recovery After Photobleaching G2 Phase / physiology Microscopy, Confocal Nucleocapsid Proteins / genetics, metabolism* Protein Transport / physiology Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Nucleocapsid Proteins; 0/nucleocapsid protein, Coronavirus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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