Document Detail

Cell cycle dependent morphology changes and associated mitochondrial DNA redistribution in mitochondria of human cell lines.
MedLine Citation:
PMID:  16120295     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Mitochondria of osteosarcoma cells (143B) in culture have variable morphologies, classified according to the shape and size of the organelle as reticular, fragmented or intermediate. Synchronization and release from G0 has shown that the morphology of mitochondria oscillates between the reticular and fragmented state in a cell cycle dependent manner. Cells in G1 have reticular mitochondria while those in S phase have fragmented mitochondria. By using a novel method of fluorescence in situ hybridization, the morphology of mitochondria was correlated with mitochondrial DNA distribution. MtDNA molecules were seen in clusters of two to four along mitochondrial filaments. In the fully fragmented state, each mitochondrion contained at least one cluster. We discuss the importance of fission and fusion events in regulating the morphology of mitochondria, segregation of mtDNA and maintenance of the organelle's functional unity.
Daciana H Margineantu; W Gregory Cox; Linda Sundell; Steven W Sherwood; Joseph M Beechem; Roderick A Capaldi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mitochondrion     Volume:  1     ISSN:  1567-7249     ISO Abbreviation:  Mitochondrion     Publication Date:  2002 May 
Date Detail:
Created Date:  2005-08-25     Completed Date:  2005-10-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100968751     Medline TA:  Mitochondrion     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  425-35     Citation Subset:  -    
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA.
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