| Cell cycle-dependent modulation of telomerase activity in tumor cells. | |
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MedLine Citation:
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PMID: 8650224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase is a ribonucleoprotein complex that is thought to add telomeric repeats onto the ends of chromosomes during the replicative phase of the cell cycle. We tested this hypothesis by arresting human tumor cell lines at different stages of the cell cycle. Induction of quiescence by serum deprivation did not affect telomerase activity. Cells arrested at the G1/S phase of the cell cycle showed similar levels of telomerase to asynchronous cultures; progression through the S phase was associated with increased telomerase activity. The highest level of telomerase activity was detected in S-phase cells. In contrast, cells arrested at G2/M phase of the cell cycle were almost devoid of telomerase activity. Diverse cell cycle blockers, including transforming growth factor beta1 and cytotoxic agents, also caused inhibition of telomerase activity. These results establish a direct link between telomerase activity and progression through the cell cycle. |
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Authors:
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X Zhu; R Kumar; M Mandal; N Sharma; H W Sharma; U Dhingra; J A Sokoloski; R Hsiao; R Narayanan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 93 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-07-25 Completed Date: 1996-07-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6091-5 Citation Subset: IM |
Affiliation:
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Division of Oncology, Roche Research Center, Hoffman-La Roche, Inc., Nutley, NJ 07110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology Cell Cycle* Humans Telomerase / antagonists & inhibitors, metabolism* Transforming Growth Factor beta / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA 56564/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Transforming Growth Factor beta; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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