Document Detail

Cell cycle-dependent modulation of biosynthesis and stimulus-evoked release of catecholamines in PC12 pheochromocytoma cells.
MedLine Citation:
PMID:  2870133     Owner:  NLM     Status:  MEDLINE    
Catecholamine biosynthesis and its stimulus-evoked release in PC12 pheochromocytoma cells were studied as a function of cell cycle by means of HPLC with electrochemical detection. We found that 3,4-dihydroxyphenylethylamine (dopamine) levels in PC12 cells remained constant throughout the period of cell cycle. In contrast, the noradrenaline content was dependent on the cell cycle: it increased during the S + G2 phase followed by a decrease in the M phase. These results were confirmed further by measuring the activities catalyzing the catecholamine biosynthesis. Thus, activities of tyrosine 3-monooxygenase and 3,4-dihydroxyphenylalanine decarboxylase were independent of the cell cycle, whereas both soluble and membrane-bound dopamine beta-monooxygenase activities were modulated during the cell cycle. On the other hand, release of the catecholamines stimulated with 50 mM KCl increased in the G1 phase, reached a maximum in the late G1, and then gradually decreased in later periods. We also found that carbamylcholine-induced release of the catecholamines occurred maximally in the early S + G2 phase followed by a decrease during the M phase. Cell cycle dependence of the catecholamine release was in good agreement with that of 45Ca2+ uptake. Thus, this study provides evidence that the catecholamine biosynthesis and its release in PC12 cells are modulated during the period of cell cycle.
T Koike; A Takashima
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  46     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-05-19     Completed Date:  1986-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1493-500     Citation Subset:  IM    
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MeSH Terms
Calcium / metabolism
Carbachol / pharmacology
Catecholamines / metabolism*
Cell Cycle*
Cell Line
Chromatography, High Pressure Liquid
Dopa Decarboxylase / metabolism
Dopamine / metabolism
Dopamine beta-Hydroxylase / metabolism
Norepinephrine / metabolism
Pheochromocytoma / metabolism*
Potassium / pharmacology
Tyrosine 3-Monooxygenase / metabolism
Reg. No./Substance:
0/Catecholamines; 51-41-2/Norepinephrine; 51-83-2/Carbachol; 7440-09-7/Potassium; 7440-70-2/Calcium; EC 3-Monooxygenase; EC beta-Hydroxylase; EC 4.1.1.-/Dopa Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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