Document Detail

Cell cycle-dependent modulation of O6-methylguanine-DNA methyltransferase in C3H/10T1/2 cells.
MedLine Citation:
PMID:  3698207     Owner:  NLM     Status:  MEDLINE    
O6-methylguanine-DNA methyltransferase (MGMT) was measured in partially synchronized cultures of C3H/10T1/2 mouse embryo cells as a function of cell cycle. The degree of synchrony and progression of the cell cycle were monitored by flow cytometry. The MGMT level was significantly reduced prior to the onset of S-phase. This reduction was concomitant with the inhibition of in vivo repair of O6-methylguanine in DNA of S-phase cells as observed earlier. The recovery of the MGMT level paralleled the progression of synchronized cells into G2. S-phase cells purified by cell sorting contained approximately 15% of the MGMT present in G0 or early G1 cells. A comparison of the in vivo repair of O6-methylguanine and MGMT levels suggests that the lack of repair of O6-methylguanine in DNA of the mouse embryo cells is due only in part to a temporal loss of MGMT.
W C Dunn; R S Foote; R E Hand; S Mitra
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  7     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-06-18     Completed Date:  1986-06-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  807-12     Citation Subset:  IM    
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MeSH Terms
Cell Cycle / drug effects
Cells, Cultured
DNA Repair
Embryo, Mammalian
Guanine / analogs & derivatives,  metabolism
Methyltransferases / analysis*
Mice, Inbred C3H
O(6)-Methylguanine-DNA Methyltransferase
Grant Support
Reg. No./Substance:
20535-83-5/O-(6)-methylguanine; 70-25-7/Methylnitronitrosoguanidine; 73-40-5/Guanine; EC 2.1.1.-/Methyltransferases; EC Methyltransferase

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