Document Detail

Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme-like cells in Xenopus embryo.
MedLine Citation:
PMID:  24167714     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Maternal Embryonic Leucine zipper Kinase (MELK) was recently shown to be involved in cell division of Xenopus embryo epithelial cells. The cytokinetic furrow of these cells ingresses asymmetrically and is developmentally regulated. Two subpopulations of xMELK, the mMELK (for "mitotic" xMELK) and iMELK ("interphase" xMELK), which differ in their spatial and temporal regulation, are detected in Xenopus embryo. How cells regulate these two xMELK populations is unknown. In this study we show that, in epithelial cells, xMELK is present at a higher concentration at the apical junctional complex, in contrast to mesenchyme-like cells, which have uniform distribution of cortical MELK. Interestingly, mMELK and iMELK also differ by their requirements towards cell-cell contacts to establish their proper cortical localization both in epithelial and mesenchyme-like cells. Receptor for Activated protein Kinase C (RACK1), which we identified as an xMELK partner, co-localizes with xMELK at the tight junction. Moreover, a truncated RACK1 construct interferes with iMELK localization at cell-cell contacts. Collectively, our results suggest that iMELK and RACK1 are present in the same complex and that RACK1 is involved in the specific recruitment of iMELK at the apical junctional complex in epithelial cells of Xenopus embryos.
Isabelle Chartrain; Yann Le Page; Guillaume Hatte; Roman Körner; Jacek Z Kubiak; Jean-Pierre Tassan
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Publication Detail:
Type:  Journal Article     Date:  2013-08-21
Journal Detail:
Title:  Biology open     Volume:  2     ISSN:  2046-6390     ISO Abbreviation:  Biol Open     Publication Date:  2013  
Date Detail:
Created Date:  2013-10-29     Completed Date:  2013-10-29     Revised Date:  2014-01-24    
Medline Journal Info:
Nlm Unique ID:  101578018     Medline TA:  Biol Open     Country:  England    
Other Details:
Languages:  eng     Pagination:  1037-48     Citation Subset:  -    
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