Document Detail

Cell cycle-dependent expression of volume-activated chloride currents in nasopharyngeal carcinoma cells.
MedLine Citation:
PMID:  12225994     Owner:  NLM     Status:  MEDLINE    
Patch-clamping and cell image analysis techniques were used to study the expression of the volume-activated Cl(-) current, I(Cl(vol)), and regulatory volume decrease (RVD) capacity in the cell cycle in nasopharyngeal carcinoma cells (CNE-2Z). Hypotonic challenge caused CNE-2Z cells to swell and activated a Cl(-) current with a linear conductance, negligible time-dependent inactivation, and a reversal potential close to the Cl(-) equilibrium potential. The sequence of anion permeability was I(-) > Br(-) > Cl(-) > gluconate. The Cl(-) channel blockers tamoxifen, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), and ATP inhibited I(Cl(vol)). Synchronous cultures of cells were obtained by the mitotic shake-off technique and by a double chemical-block (thymidine and hydroxyurea) technique. The expression of I(Cl(vol)) was cell cycle dependent, being high in G(1) phase, downregulated in S phase, but increasing again in M phase. Hypotonic solution activated RVD, which was cell cycle dependent and inhibited by the Cl(-) channel blockers NPPB, tamoxifen, and ATP. The expression of I(Cl(vol)) was closely correlated with the RVD capacity in the cell cycle, suggesting a functional relationship. Inhibition of I(Cl(vol)) by NPPB (100 microM) arrested cells in G(0)/G(1). The data also suggest that expression of I(Cl(vol)) and RVD capacity are actively modulated during the cell cycle. The volume-activated Cl(-) current associated with RVD may therefore play an important role during the cell cycle progress.
Lixin Chen; Liwei Wang; Linyan Zhu; Sihai Nie; Jin Zhang; Ping Zhong; Bo Cai; Haibing Luo; Tim J C Jacob
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  283     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-12     Completed Date:  2002-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1313-23     Citation Subset:  IM    
School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, Wales, UK.
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MeSH Terms
Adenosine Triphosphate / pharmacology
Antineoplastic Agents, Hormonal / pharmacology
Bromides / pharmacology
Carcinoma / drug therapy,  metabolism*,  pathology
Cell Cycle / drug effects,  physiology*
Cell Division / drug effects
Cell Size / drug effects
Chloride Channels / antagonists & inhibitors,  metabolism*
Chlorides / metabolism*
Gluconates / pharmacology
Hypotonic Solutions / pharmacology
Iodides / pharmacology
Membrane Potentials / drug effects
Nasopharyngeal Neoplasms / drug therapy,  metabolism*,  pathology
Nitrobenzoates / pharmacology
Patch-Clamp Techniques
Permeability / drug effects
Tamoxifen / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents, Hormonal; 0/Bromides; 0/Chloride Channels; 0/Chlorides; 0/Gluconates; 0/Hypotonic Solutions; 0/Iodides; 0/Nitrobenzoates; 10540-29-1/Tamoxifen; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 526-95-4/gluconic acid; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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