Document Detail

Cell cycle-dependent expression of a glioma-specific chloride current: proposed link to cytoskeletal changes.
MedLine Citation:
PMID:  9357773     Owner:  NLM     Status:  MEDLINE    
We recently demonstrated expression of a novel, glioma-specific Cl- current in glial-derived tumor cells (gliomas), including stable cell lines such as STTG1, derived from a human anaplastic astrocytoma. We used STTG1 cells to study whether glioma Cl- channel (GCC) activity is regulated during cell cycle progression. Cells were arrested in defined stages of cell cycle (G0, G1, G1/S, S, and M phases) using serum starvation, mevastatin, hydroxyurea, demecolcine, and cytosine beta-D-arabinofuranoside. Cell cycle arrest was confirmed by measuring [3H]thymidine incorporation and by DNA flow cytometry. Using whole cell patch-clamp recordings, we demonstrate differential changes in GCC activity after cell proliferation and cell cycle progression was selectively altered; specifically, channel expression was low in serum-starved, G0-arrested cells, increased significantly in early G1, decreased during S phase, and increased after arrest in M phase. Although the link between the cell cycle and GCC activity is not yet clear, we speculate that GCCs are linked to the cytoskeleton and that cytoskeletal rearrangements associated with cell division lead to the observed changes in channel activity. Consistent with this hypothesis, we demonstrate the activation of GCC by disruption of F-actin using cytochalasin D or osmotic cell swelling.
N Ullrich; H Sontheimer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-21     Completed Date:  1997-11-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1290-7     Citation Subset:  IM    
Department of Neurobiology, University of Alabama at Birmingham, 35294, USA.
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MeSH Terms
Actins / ultrastructure
Cell Cycle / physiology*
Cell Division
Cell Line
Chloride Channels / biosynthesis*
Cytoskeleton / pathology,  ultrastructure*
Flow Cytometry
Glioblastoma / pathology,  physiopathology*
Hypotonic Solutions
Membrane Potentials
Patch-Clamp Techniques
Thymidine / metabolism
Tumor Cells, Cultured
Grant Support
R01-31234//PHS HHS
Reg. No./Substance:
0/Actins; 0/Chloride Channels; 0/Hypotonic Solutions; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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