| Cell cycle-dependent expression of a glioma-specific chloride current: proposed link to cytoskeletal changes. | |
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MedLine Citation:
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PMID: 9357773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently demonstrated expression of a novel, glioma-specific Cl- current in glial-derived tumor cells (gliomas), including stable cell lines such as STTG1, derived from a human anaplastic astrocytoma. We used STTG1 cells to study whether glioma Cl- channel (GCC) activity is regulated during cell cycle progression. Cells were arrested in defined stages of cell cycle (G0, G1, G1/S, S, and M phases) using serum starvation, mevastatin, hydroxyurea, demecolcine, and cytosine beta-D-arabinofuranoside. Cell cycle arrest was confirmed by measuring [3H]thymidine incorporation and by DNA flow cytometry. Using whole cell patch-clamp recordings, we demonstrate differential changes in GCC activity after cell proliferation and cell cycle progression was selectively altered; specifically, channel expression was low in serum-starved, G0-arrested cells, increased significantly in early G1, decreased during S phase, and increased after arrest in M phase. Although the link between the cell cycle and GCC activity is not yet clear, we speculate that GCCs are linked to the cytoskeleton and that cytoskeletal rearrangements associated with cell division lead to the observed changes in channel activity. Consistent with this hypothesis, we demonstrate the activation of GCC by disruption of F-actin using cytochalasin D or osmotic cell swelling. |
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Authors:
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N Ullrich; H Sontheimer |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 273 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-11-21 Completed Date: 1997-11-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C1290-7 Citation Subset: IM |
Affiliation:
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Department of Neurobiology, University of Alabama at Birmingham, 35294, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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ultrastructure Cell Cycle / physiology* Cell Division Cell Line Chloride Channels / biosynthesis* Cytoskeleton / pathology, ultrastructure* Flow Cytometry Glioblastoma / pathology, physiopathology* Humans Hypotonic Solutions Membrane Potentials Patch-Clamp Techniques Thymidine / metabolism Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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R01-31234//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Chloride Channels; 0/Hypotonic Solutions; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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