| Cell cycle-dependent expression of cyclooxygenase-2 in human fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 11156939 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The purpose of this investigation is to determine whether the levels of cyclooxygenase-2 (COX-2) expression are cell cycle dependent. We used a serum-starved human foreskin fibroblast model to determine changes in COX-2 mRNA, protein, and promoter activity in response to stimulation with interleukin-1b (IL-1b) and phorbol 12-myristate 13-acetate (PMA) at G0, G1, S and G2/M phases of the cell cycle. IL-1b (1 ng/ml) and PMA (100 nM) induced robust COX-2 expression in the G0 cells, and the level of COX-2 expression declined progressively after the cells had entered the cell cycle. The COX-2 mRNA level at G1, S and G2/M phases of the cell cycle was 76%, 46%, and 30% of that at G0, respectively. A 5-flanking promoter fragment of COX-2 constructed into a luciferase expression vector was transfected into cells. The promoter activity in response to PMA stimulation was significantly higher in G0 than in S phase cells. These results imply that G0 cells are the key players in inflammation and other COX-2-dependent pathophysiological processes. When the cells are in the proliferative phase, COX-2 inducibility becomes restrained probably by an endogenous control mechanism to avoid COX-2 mediated oxidative DNA damage. |
| | |
Authors:
|
D W Gilroy; M A Saunders; L Sansores-Garcia; N Matijevic-Aleksic; K K Wu |
Related Documents
:
|
8352539 - Cell cycle related uptake, retention and toxicity of idarubicin, daunorubicin and doxor... 1361489 - Activin a-induced differentiation in k562 cells is associated with a transient hypophos... 8227179 - Arrest of cell cycle progression of hela cells in the early g1 phase in k(+)-depleted c... 2549409 - Comparison of gamma-radiation-induced accumulation of ataxia telangiectasia and control... 1702269 - Ethanol causes accelerated g1 arrest in differentiating hl-60 cells. 11054109 - Effect of cadmium on the relationship between replicative and repair dna synthesis in s... 19403949 - Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cel... 7579799 - Cd23 molecule acts as a galactose-binding lectin in the cell aggregation of ebv-transfo... 1491599 - Determination of beta-carotene in plasma, blood cells and buccal mucosa by electrochemi... |
Publication Detail:
|
Type: Journal Article Date: 2000-12-08 |
Journal Detail:
|
Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 15 ISSN: 0892-6638 ISO Abbreviation: FASEB J. Publication Date: 2001 Feb |
Date Detail:
|
Created Date: 2001-02-22 Completed Date: 2001-03-22 Revised Date: 2012-02-15 |
Medline Journal Info:
|
Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
|
Languages: eng Pagination: 288-90 Citation Subset: IM |
Affiliation:
|
Vascular Biology Research Center and Division of Hematology, Department of Medicine, University of Texas-Houston Medical School, Houston, Texas 77030-1503, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle
/
physiology* Cells, Cultured Cyclooxygenase 2 Fibroblasts / cytology*, enzymology* G0 Phase Gene Expression Regulation, Enzymologic / drug effects, physiology* Humans Isoenzymes / genetics*, metabolism Membrane Proteins Models, Biological Promoter Regions, Genetic / drug effects Prostaglandin-Endoperoxide Synthases / genetics*, metabolism S Phase Skin / cytology Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/Membrane Proteins; 16561-29-8/Tetradecanoylphorbol Acetate; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic requirement of p47phox for superoxide production by murine microglia.
Next Document: Effects of dietary calcium on adipocyte lipid metabolism and body weight regulation in energy-restri...