| Cell cycle dependent changes in potassium transport. | |
| | |
MedLine Citation:
|
PMID: 956277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
K transport has been investigated during progression of cultured Ehrlich ascites tumor cells through the cell cycle. Using a double thymidine block technique, Ehrlich cells carried in continuous culture have been synchronized, as verified by simultaneous monitoring of cell number, cell volume, 3H-thymidine incorporation and mitotic index. Unidirectional influx, efflux and cell content of K have been monitored throughout the cell cycle. The nature of the pump mediated, ouabain-sensitive K flux and the furosemide-sensitive component of K flux, presumably representing K-K exchange, have also been evaluated. In early S period the ouabain sensitive component, representing the Na-K pump, comprises 52% of the total unidirectional K influx and subsequently declines during G2 period to a minimum of 40% in mid G2. During M and early S the activity again rises. As the ouabain sensitive component becomes maximal in late S period, the furosemide sensitive component diminishes from approximately 30% of the total influx to approximately 10%. The same pattern is observed in the G2 period. As the pump component diminishes, the furosemide sensitive component increases. Furosemide sensitive K efflux has also been monitored and the pattern is equivalent to that observed in the invlux studies. No change in net K flux is observed in the presence of furosemide. This indicates that the furosemide sensitive component represents an exchange component for K. These results are consistent with the conclusion that the alterations in exchange and pump fluxes are physiological events associated with progression of the cell cycle. |
| | |
Authors:
|
B Mills; J T Tupper |
Related Documents
:
|
1933367 - Effects of relative hypoxia and hypercapnia on intracellular ph and membrane potential ... 8873677 - Regional differences in electrophysiological properties of epicardium, midmyocardium, a... 9387117 - Effect of cellular hydration on protein metabolism. 15093387 - Changes in gill morphology of atlantic salmon (salmo salar) smolts due to addition of a... 19562367 - Arginine transport in human erythroid cells: discrimination of cat1 and 4f2hc/y+lat2 ro... 22025527 - Syk inhibition and response prediction in diffuse large b-cell lymphoma. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 89 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1976 Sep |
Date Detail:
|
Created Date: 1976-11-01 Completed Date: 1976-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 123-32 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport, Active
/
drug effects Cell Division* Cell Line Cell Membrane / metabolism* Furosemide / pharmacology Kinetics Mitosis Ouabain / pharmacology Potassium / metabolism* Sodium / metabolism |
| Chemical | |
Reg. No./Substance:
|
54-31-9/Furosemide; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Density-dependent regulation of proliferation rate in cultured, androgen-responsive, tumour cells.
Next Document: Reversible independent alterations in glucose transport and metabolism in cultured human cells depri...