| Cell cycle-dependent calcium oscillations in mouse embryonic stem cells. | |
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MedLine Citation:
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PMID: 17092997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During cell cycle progression, somatic cells exhibit different patterns of intracellular Ca(2+) signals during the G(0) phase, the transition from G(1) to S, and from G(2) to M. Because pluripotent embryonic stem (ES) cells progress through cell cycle without the gap phases G(1) and G(2), we aimed to determine whether mouse ES (mES) cells still exhibit characteristic changes of intracellular Ca(2+) concentration during cell cycle progression. With confocal imaging of the Ca(2+)-sensitive dye fluo-4 AM, we identified that undifferentiated mES cells exhibit spontaneous Ca(2+) oscillations. In control cultures where 50.4% of the cells reside in the S phase of the cell cycle, oscillations appeared in 36% of the cells within a colony. Oscillations were not initiated by Ca(2+) influx but depended on inositol 1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release and the refilling of intracellular stores by a store-operated Ca(2+) influx (SOC) mechanism. Using cell cycle synchronization, we determined that Ca(2+) oscillations were confined to the G(1)/S phase ( approximately 70% oscillating cells vs. G(2)/M with approximately 15% oscillating cells) of the cell cycle. ATP induced Ca(2+) oscillations, and activation of SOC could be induced in G(1)/S and G(2)/M synchronized cells. Intracellular Ca(2+) stores were not depleted, and all three IP(3) receptor isoforms were present throughout the cell cycle. Cell cycle analysis after EGTA, BAPTA-AM, 2-aminoethoxydiphenyl borate, thapsigargin, or U-73122 treatment emphasized that IP(3)-mediated Ca(2+) release is necessary for cell cycle progression through G(1)/S. Because the IP(3) receptor sensitizer thimerosal induced Ca(2+) oscillations only in G(1)/S, we propose that changes in IP(3) receptor sensitivity or basal levels of IP(3) could be the basis for the G(1)/S-confined Ca(2+) oscillations. |
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Authors:
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Nidhi Kapur; Gregory A Mignery; Kathrin Banach |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-11-08 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 292 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-12 Completed Date: 2007-06-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C1510-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, Stritch School of Medicine, Loyola University Chicago, 2160 South First Ave., Maywood, IL 60153, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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antagonists & inhibitors Animals Boron Compounds / pharmacology Calcium / metabolism* Calcium Signaling* Cell Cycle / drug effects, physiology* Cells, Cultured Chelating Agents / pharmacology Egtazic Acid / analogs & derivatives, pharmacology Embryonic Stem Cells / drug effects, physiology* Estrenes / pharmacology Inositol 1,4,5-Trisphosphate / physiology Inositol 1,4,5-Trisphosphate Receptors / antagonists & inhibitors, metabolism Mice Protein Isoforms / antagonists & inhibitors, metabolism Pyrrolidinones / pharmacology Thapsigargin / pharmacology Type C Phospholipases / antagonists & inhibitors |
| Grant Support | |
ID/Acronym/Agency:
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MH-53367/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/2-aminoethoxydiphenyl borate; 0/Boron Compounds; 0/Chelating Agents; 0/Estrenes; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Protein Isoforms; 0/Pyrrolidinones; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 139890-68-9/1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; 67-42-5/Egtazic Acid; 67526-95-8/Thapsigargin; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 3.1.4.-/Type C Phospholipases; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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