Document Detail

Cell cycle-dependent association of Rad51 with the nuclear matrix.
MedLine Citation:
PMID:  17263595     Owner:  NLM     Status:  MEDLINE    
Progression of the cells through the S phase of the cell cycle is connected with accumulation of stalled and collapsed replication forks that are repaired by homologous recombination. To investigate the temporal order of homologous recombination events during the S phase, HeLa cells synchronized at the G1/S phase boundary with mimosine were released to progress into the S phase and the phosphorylation of the histone variant H2AX, the appearance of Rad51 nuclear foci and the subcellular redistribution of Rad51 were followed. The results showed that there was gradual accumulation of double-strand breaks as judged by the increase in the phosphorylation of H2AX during the S phase. Rad51 nuclear foci did not appear until middle S phase, and this was accompanied by an increase in the chromatin- and nuclear matrix-bound Rad51 in the middle to late S phase. To determine the role of the intra S phase checkpoint in the S phase-dependent redistribution of Rad51 the cells were released in the S phase in the presence of the protein kinase inhibitors caffeine and wortmannin. The results suggest that the association of Rad51 with the nuclear matrix is regulated by activation of the intra S phase ATR-dependent checkpoint pathway.
Emil Mladenov; Irina Tsaneva; Boyka Anachkova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA and cell biology     Volume:  26     ISSN:  1044-5498     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-03-01     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-43     Citation Subset:  IM    
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
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MeSH Terms
Cell Cycle Proteins / physiology
Cell Nucleus / metabolism
DNA / biosynthesis
DNA Breaks, Double-Stranded
DNA Replication
DNA-Binding Proteins / physiology
Hela Cells
Histones / metabolism*
Nuclear Matrix / physiology*
Protein-Serine-Threonine Kinases / physiology
Rad51 Recombinase / metabolism,  physiology*
S Phase / physiology*
Tumor Suppressor Proteins / physiology
Grant Support
070397//Wellcome Trust
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/H2AFX protein, human; 0/Histones; 0/Tumor Suppressor Proteins; 9007-49-2/DNA; EC 2.7.1.-/ATR protein, human; EC Kinases; EC telangiectasia mutated protein; EC 2.7.7.-/RAD51 protein, human; EC 2.7.7.-/Rad51 Recombinase

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