| Cell-cycle-dependent Xenopus TRF1 recruitment to telomere chromatin regulated by Polo-like kinase. | |
| | |
MedLine Citation:
|
PMID: 16424898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Telomeres are regulated by a homeostatic mechanism that includes telomerase and telomeric repeat binding proteins, TRF1 and TRF2. Recently, it has been hypothesized that telomeres assume distinct configurations in a cell-cycle-dependent manner, although direct biochemical evidence is lacking. Here we demonstrated that Xenopus TRF1 (xTRF1) associates with telomere chromatin specifically in mitotic Xenopus egg extracts, and dissociates from it upon mitotic exit. Both the N-terminal TRF-homology (TRFH) domain and the linker region connecting the TRFH domain and the C-terminal Myb domain are required for this cell-cycle-dependent association of xTRF1 with chromatin. In contrast, Xenopus TRF2 (xTRF2) associates with chromatin throughout the cell cycle. We showed that Polo-like kinase (Plx1) phosphorylates xTRF1 in vitro. Moreover, the mitotic xTRF1-chromatin association was significantly impaired when Plx1 was immunodepleted from the extracts. Finally, high telomerase activities were detected in association with replicating interphase chromatin compared with mitotic chromatin. These results indicate that telomere chromatin is actively regulated by cell-cycle-dependent processes, and provide an insight for understanding how telomeres undergo DNA metabolisms during the cell cycle. |
| | |
Authors:
|
Atsuya Nishiyama; Keiko Muraki; Motoki Saito; Keita Ohsumi; Takeo Kishimoto; Fuyuki Ishikawa |
Related Documents
:
|
3740938 - Lymphocyte proliferation and nucleoid sedimentation in a case of premature aging distin... 20364118 - Hrad21 overexpresses and localizes to the alt-associated promyelocytic leukemia body in... 19427898 - Early onset senescence occurs when fibroblasts lack the glutamate-cysteine ligase modif... 10982178 - Incidence of mosaic cell lines in vivo and malsegregation of chromosome 21 in lymphocyt... 12016218 - Phosphatidylserine binding of class b scavenger receptor type i, a phagocytosis recepto... 17269748 - The phosphorus source phytate changes the composition of the cell wall proteome in baci... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-19 |
Journal Detail:
|
Title: The EMBO journal Volume: 25 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2006 Feb |
Date Detail:
|
Created Date: 2006-02-08 Completed Date: 2006-03-28 Revised Date: 2011-11-02 |
Medline Journal Info:
|
Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
|
Languages: eng Pagination: 575-84 Citation Subset: IM |
Affiliation:
|
Laboratory of Cell Cycle Regulation, Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals CDC2 Protein Kinase / metabolism Cell Cycle* Cell Cycle Proteins / metabolism* Chromatin / metabolism* Mitosis Ovum / metabolism Phosphorylation Protein Binding Protein Structure, Tertiary Protein-Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / metabolism* Telomerase / metabolism Telomere / metabolism* Telomeric Repeat Binding Protein 1 / metabolism* Telomeric Repeat Binding Protein 2 / metabolism Xenopus Proteins / metabolism* Xenopus laevis |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/Chromatin; 0/Proto-Oncogene Proteins; 0/Telomeric Repeat Binding Protein 1; 0/Telomeric Repeat Binding Protein 2; 0/Xenopus Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and ma...
Next Document: The Ca2+-activated cation channel TRPM4 is regulated by phosphatidylinositol 4,5-biphosphate.