| Cell-cycle-dependent PC-PLC regulation by APC/C(Cdc20)-mediated ubiquitin-proteasome pathway. | |
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MedLine Citation:
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PMID: 19347873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphatidylcholine-specific phospholipase C (PC-PLC) is involved in the cell signal transduction, cell proliferation, and apoptosis. The mechanism of its action, however, has not been fully understood, particularly, the role of PC-PLC in the cell cycle. In the present study, we found that cell division cycle 20 homolog (Cdc20) and PC-PLC were co-immunoprecipitated reciprocally by either antibody in rat hepatoma cells CBRH-7919 as well as in rat liver tissue. Using confocal microscopy, we found that PC-PLC and Cdc20 were co-localized in the perinuclear endoplasmic reticulum region (the "juxtanuclear quality control" compartment, JUNQ). The expression level and activities of PC-PLC changed in a cell-cycle-dependent manner and were inversely correlated with the expression of Cdc20. Intriguingly, Cdc20 overexpression altered the subcellular localization and distribution of PC-PLC, and caused PC-PLC degradation by the ubiquitin proteasome pathway (UPP). Taken together, our data indicate that PC-PLC regulation in cell cycles is controlled by APC/C(Cdc20)-mediated UPP. |
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Authors:
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Da Fu; Yushui Ma; Wei Wu; Xuchao Zhu; Chengyou Jia; Qianlei Zhao; Chunyi Zhang; Xing Zhong Wu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 107 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-30 Completed Date: 2009-10-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 686-96 Citation Subset: IM |
Copyright Information:
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2009 Wiley-Liss, Inc. |
Affiliation:
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Department of Biochemistry, Fudan University, Shanghai, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Cell Cycle* Cell Cycle Proteins / analysis, physiology* Cell Line Cercopithecus aethiops Endoplasmic Reticulum / chemistry Liver / chemistry Proteasome Endopeptidase Complex / metabolism* Rats Type C Phospholipases / analysis, metabolism* Ubiquitin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cdc20 protein, rat; 0/Cell Cycle Proteins; 0/Ubiquitin; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.3/phosphatidylcholine-specific phospholipase C; EC 3.4.25.1/Proteasome Endopeptidase Complex |
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