Document Detail


Cell cycle dependence of elicitor-induced signal transduction in tobacco BY-2 cells.
MedLine Citation:
PMID:  15659447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular links between the cell cycle and defense responses in plants are largely unknown. Using synchronized tobacco BY-2 cells, we analyzed the cell cycle dependence of elicitor-induced defense responses. In synchronized cultured apoaequorin-expressing cells, the increase in cytosolic free Ca2+ induced by a proteinaceous elicitor, cryptogein, was greatly suppressed during the G2 and M phases in comparison with G1 or S phases. Treatment with cryptogein during the G1 or S phases also induced biphasic (rapid/transient and slow/prolonged) responses in activation of mitogen-activated protein kinases (MAPKs) and production of reactive oxygen species (ROS). In contrast, elicitor treatment during the G2 or M phases induced only a rapid and transient phase of MAPK activation and ROS production. Their slow and prolonged phases as well as expression of defense-related genes, cell cycle arrest and cell death were induced only after the cell cycle progressed to the G1 phase; removal of the elicitor before the start of the G1 phase inhibited these responses. These results suggest that although cryptogein recognition occurred at all phases of the cell cycle, the recognition during the S or G1 phases, but not at the G2 or M phases, induces the prolonged activation of MAPKs and the prolonged production of ROS, followed by cell cycle arrest, accumulation of defense-related gene transcripts and cell death. Elicitor signal transduction depends on the cell cycle and is regulated differently at each phase.
Authors:
Yasuhiro Kadota; Takashi Watanabe; Shinsuke Fujii; Yutaka Maeda; Ryoko Ohno; Katsumi Higashi; Toshio Sano; Shoshi Muto; Seiichiro Hasezawa; Kazuyuki Kuchitsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-19
Journal Detail:
Title:  Plant & cell physiology     Volume:  46     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-02-09     Completed Date:  2005-04-19     Revised Date:  2012-07-20    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  156-65     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan.
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MeSH Terms
Descriptor/Qualifier:
Aequorin / metabolism
Algal Proteins / pharmacology
Calcium Signaling / drug effects
Cell Cycle / drug effects
Extracellular Signal-Regulated MAP Kinases / metabolism
MAP Kinase Signaling System / drug effects
Reactive Oxygen Species / metabolism
Signal Transduction / drug effects
Tobacco / cytology*,  drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Algal Proteins; 0/Reactive Oxygen Species; 0/cryptogein protein, Phytophthora cryptogea; 50934-79-7/Aequorin; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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