| Cell cycle control of c-kit+IL-7R+ B precursor cells by two distinct signals derived from IL-7 receptor and c-kit in a fully defined medium. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 7543134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An important goal for the investigation of the proliferation of mammalian cells is to establish a fully defined condition for culturing them in vitro. Here, we report establishment of a fully defined culture condition that supports the primary culture of normal c-kit+IL-7 receptor (IL-7R)+ B precursor cells without the aid of stromal cell lines. This defined culture condition contains IL-7, the ligand for c-kit, transferrin, insulin, and bovine serum albumin as protein components. By using the cell lines derived from RAG2(-/-) mice, which do not differentiate into c-kit- stage, we have evaluated the role of each protein in the cell cycle progression of c-kit+IL-7R+ B precursor cells. Since B precursor cells can grow without insulin, c-kit remains a sole functional receptor tyrosine kinase for their growth. While both c-kit ligand (KL) and IL-7 are the requisite molecules for sustained proliferation of B precursor cells, each molecule plays distinct roles. IL-7 starvation results in prompt arrest of the cells at G1. An accumulation of the cells in the mitotic phase was also detected. Thus, the major role of IL-7 is to regulate the G1/S transition and the process of cytokinesis of B precursor cells. Although prolonged KL starvation over 48 h resulted in accumulation of G1 cells, its effect could not be detected within 24 h, which is long enough for all the cells to complete one cell cycle. This suggests that KL might be involved in the cell cycle progression of B precursor cells in a manner that its signal could still be effective in the one or two cell cycles that follow. Although molecular nature of the signals underlying the present observation awaits future investigation, the method described in this report would provide a useful model system for investigating the signaling pathways that are involved in the cell cycle progression of B precursor cells. |
| | |
Authors:
|
M Yasunaga; F Wang; T Kunisada; S Nishikawa; S Nishikawa |
Related Documents
:
|
2899074 - Isoprenoid synthesis during the cell cycle. studies of 3-hydroxy-3-methylglutaryl-coenz... 6825154 - Spermatogonial multiplication in the chinese hamster. ii. cell cycle properties of undi... 21510844 - Hepatocytic differentiation of rhesus monkey embryonic stem cells promoted by collagen ... 8711204 - Genetic control of mitosis, meiosis and cellular differentiation during mammalian sperm... 21290054 - Correlation of p16(ink4a) expression and hpv copy number with cellular ftir spectroscop... 12102174 - Synergistic effect of vitamin d derivatives and retinoids on c2c12 skeletal muscle cells. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of experimental medicine Volume: 182 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1995 Aug |
Date Detail:
|
Created Date: 1995-09-07 Completed Date: 1995-09-07 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 315-23 Citation Subset: IM |
Affiliation:
|
Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD / physiology* B-Lymphocytes / cytology* Cell Cycle* Cells, Cultured Growth Substances / pharmacology Hematopoiesis / drug effects Hematopoietic Cell Growth Factors / physiology* Immunophenotyping Interleukin-7 / physiology* Mice Mice, Inbred C57BL Mice, Knockout Proto-Oncogene Proteins / physiology* Proto-Oncogene Proteins c-kit Receptor Protein-Tyrosine Kinases / physiology* Receptors, Colony-Stimulating Factor / physiology* Receptors, Interleukin / physiology* Receptors, Interleukin-7 Signal Transduction Stem Cell Factor |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD; 0/Growth Substances; 0/Hematopoietic Cell Growth Factors; 0/Interleukin-7; 0/Proto-Oncogene Proteins; 0/Receptors, Colony-Stimulating Factor; 0/Receptors, Interleukin; 0/Receptors, Interleukin-7; 0/Stem Cell Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 8 Year: 1995 Volume: 182 Issue: 2 First Page: 315 Last Page: 323 ID: 2192150 Publisher Id: 95355831 PubMed Id: 7543134 |
| Cell cycle control of c-kit+IL-7R+ B precursor cells by two distinct signals derived from IL-7 receptor and c-kit in a fully defined medium | |
Article Categories:
|
|
Previous Document: Effects on glucose tolerance, insulin secretion, insulin-like growth factor 1 and its binding protei...
Next Document: Prevention of experimental allergic encephalomyelitis in rats by targeting autoantigen to B cells: e...