| Cell cycle control of β-cell replication in the prenatal and postnatal human pancreas. | |
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MedLine Citation:
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PMID: 20978233 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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β-Cell regeneration declines with aging, but the molecular mechanisms controlling β-cell replication in humans are not well understood. We compared the expression of selected cell cycle proteins in prenatal and adult tissue and examined the association of these proteins with β-cell replication. Pancreatic tissue from a total of 20 human fetuses and adults was stained for Ki67, cyclin D3, p16 and p27, and insulin. The β-cellular expression of these cell cycle proteins was determined. The frequency of β-cell replication was lower in adult compared with prenatal β-cells (<0.5 vs. 3.4 ± 0.5%, respectively; P < 0.0001). p16 was sporadically expressed in prenatal β-cells (8.0 ± 1.1%) but highly enriched in adult β-cells (63.1 ± 5.2%, P < 0.0001). Likewise, the expression of p27 was much lower in prenatal β-cells (1.7 ± 0.4 vs. 44.1 ± 5.4%, respectively, P < 0.0001), and cyclin D3 expression increased from 24.2 ± 4.1 to 47.25 ± 5.0%, respectively (P < 0.001), with aging. The expression of all three proteins was significantly correlated with each other (P < 0.01 and r > 0.75, respectively). The strong expression of cyclin D3 in adult human β-cells and its correlation to p27 and p16 suggest a positive role in human β-cell cycle regulation. p16 and p27 appear to restrict β-cell replication with aging. The age dependency of cell cycle regulation in human β-cells might explain the reduced β-cell regeneration in adult humans. |
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Authors:
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Christina U Köhler; Marvin Olewinski; Andrea Tannapfel; Wolfgang E Schmidt; Helga Fritsch; Juris J Meier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-26 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 300 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-29 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E221-30 Citation Subset: IM |
Affiliation:
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Department of Medicine I, St. Josef-Hospital, Ruhr-University of Bochum, Gudrunstr. 56, 44791 Bochum, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Cell Cycle* Cell Cycle Proteins / metabolism* Cell Division Cyclin D3 / metabolism Cyclin-Dependent Kinase Inhibitor p16 / metabolism Cyclin-Dependent Kinase Inhibitor p27 / metabolism Female Gestational Age Humans Immunohistochemistry Insulin / metabolism Insulin-Secreting Cells / cytology*, metabolism* Ki-67 Antigen / metabolism Male Middle Aged Pancreas / cytology, embryology*, growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/CCND3 protein, human; 0/Cell Cycle Proteins; 0/Cyclin D3; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Ki-67 Antigen; 11061-68-0/Insulin; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27 |
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