| Cell cycle checkpoint and apoptosis induction in glioblastoma cells and fibroblasts irradiated with carbon beam. | |
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MedLine Citation:
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PMID: 17548940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/mum were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gamma-sensitivity, and yield of cell death correlated to LET values. A p53-wild-type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells. |
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Authors:
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Koji Tsuboi; Takashi Moritake; Yukihiro Tsuchida; Koichi Tokuuye; Akira Matsumura; Koichi Ando |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-05 |
Journal Detail:
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Title: Journal of radiation research Volume: 48 ISSN: 0449-3060 ISO Abbreviation: J. Radiat. Res. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-20 Completed Date: 2007-08-20 Revised Date: 2012-03-28 |
Medline Journal Info:
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Nlm Unique ID: 0376611 Medline TA: J Radiat Res Country: Japan |
Other Details:
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Languages: eng Pagination: 317-25 Citation Subset: IM |
Affiliation:
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Proton Medical Research Center, Doctoral Program in Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba. tsuboi-k@md.tsukuba.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Carbon / chemistry* Cell Cycle / radiation effects* Cell Line, Tumor Dose-Response Relationship, Drug Fibroblasts / metabolism, radiation effects* Flow Cytometry Gene Expression Regulation, Neoplastic / radiation effects* Genes, p53 Glioblastoma / radiotherapy* Humans Linear Energy Transfer Microscopy, Fluorescence Mutation Radiation, Ionizing |
| Chemical | |
Reg. No./Substance:
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7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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