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Cell cycle changes in the adenylate cyclase of C6 glioma cells.
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MedLine Citation:
PMID:  6265474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adenylate cyclase of C6 glioma cell cultures was characterized for sensitivity to the beta-adrenergic agonist isoproterenol, as well as fluoride, and GTP as a function of the cell cycle. The mitotic phase of the cell cycle was emphasized because both the basal cellular cyclic AMP level and the intact C6 cell's capacity to accumulate cyclic AMP in response to isoproterenol decreased during mitosis. Basal and stimulated adenylate cyclase activities in mitotic cells were decreased relative to the enzyme activities in the G1, S, and G2 phases of the cell cycle. Analysis of the beta-adrenergic receptor using the radioligand(-)[3H]dihydroalprenolol showed that neither ligand affinity nor receptor density changed during the cell cycle, indicating that the reduced adenylate cyclase activity of the mitotic C6 cell was not caused by alterations in this hormone receptor. The reduction in the mitotic cell's basal adenylate cyclase activity was more prominent than the decrease in isoproterenol-, fluoride, or GTP-stimulated activities suggesting that the effectiveness of these enzymes activators (i.e., the efficiency of the coupling mechanism) was not attenuated during mitosis. These studies indicate that the intrinsic catalytic capacity (not the beta-adrenergic receptor or the coupling mechanism) of the C6 adenylate cyclase complex is reduced during mitosis and contributes to the mitotic cell's inability to accumulate and maintain the cyclic AMP concentration at the interphase level.
Authors:
R F Howard; J R Sheppard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  90     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1981-09-22     Completed Date:  1981-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism*
Animals
Cell Line
Cyclic AMP / metabolism
Dihydroalprenolol / metabolism
Glioma
Guanosine Triphosphate / pharmacology
Interphase*
Mitosis*
Rats
Receptors, Adrenergic, beta / metabolism
Sodium Fluoride / pharmacology
Grant Support
ID/Acronym/Agency:
NS14436/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Adrenergic, beta; 60-92-4/Cyclic AMP; 60106-89-0/Dihydroalprenolol; 7681-49-4/Sodium Fluoride; 86-01-1/Guanosine Triphosphate; EC 4.6.1.1/Adenylate Cyclase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 7 Year: 1981
Volume: 90 Issue: 1
First Page: 169 Last Page: 175
ID: 2111841
Publisher Id: 81239790
PubMed Id: 6265474

Cell cycle changes in the adenylate cyclase of C6 glioma cells


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