| Cell cycle changes in the adenylate cyclase of C6 glioma cells. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 6265474 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The adenylate cyclase of C6 glioma cell cultures was characterized for sensitivity to the beta-adrenergic agonist isoproterenol, as well as fluoride, and GTP as a function of the cell cycle. The mitotic phase of the cell cycle was emphasized because both the basal cellular cyclic AMP level and the intact C6 cell's capacity to accumulate cyclic AMP in response to isoproterenol decreased during mitosis. Basal and stimulated adenylate cyclase activities in mitotic cells were decreased relative to the enzyme activities in the G1, S, and G2 phases of the cell cycle. Analysis of the beta-adrenergic receptor using the radioligand(-)[3H]dihydroalprenolol showed that neither ligand affinity nor receptor density changed during the cell cycle, indicating that the reduced adenylate cyclase activity of the mitotic C6 cell was not caused by alterations in this hormone receptor. The reduction in the mitotic cell's basal adenylate cyclase activity was more prominent than the decrease in isoproterenol-, fluoride, or GTP-stimulated activities suggesting that the effectiveness of these enzymes activators (i.e., the efficiency of the coupling mechanism) was not attenuated during mitosis. These studies indicate that the intrinsic catalytic capacity (not the beta-adrenergic receptor or the coupling mechanism) of the C6 adenylate cyclase complex is reduced during mitosis and contributes to the mitotic cell's inability to accumulate and maintain the cyclic AMP concentration at the interphase level. |
| | |
Authors:
|
R F Howard; J R Sheppard |
Related Documents
:
|
2988544 - Regulation of cyclic gmp levels by neurotensin in neuroblastoma clone n1e115. 6162004 - Properties of bovine oligodendroglia isolated by a new procedure using physiologic cond... 11956184 - New signaling pathway for parathyroid hormone and cyclic amp action on extracellular-re... 2983884 - Presence and regulation of tyrosinase activity in human neuroblastoma cell variants in ... 16641284 - Adeno-associated virus type 2 increases proteosome-dependent degradation of p21waf1 in ... 7522884 - Positive selection of human blood cells using improved high gradient magnetic separatio... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of cell biology Volume: 90 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1981 Jul |
Date Detail:
|
Created Date: 1981-09-22 Completed Date: 1981-09-22 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 169-75 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenylate Cyclase
/
metabolism* Animals Cell Line Cyclic AMP / metabolism Dihydroalprenolol / metabolism Glioma Guanosine Triphosphate / pharmacology Interphase* Mitosis* Rats Receptors, Adrenergic, beta / metabolism Sodium Fluoride / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
NS14436/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, Adrenergic, beta; 60-92-4/Cyclic AMP; 60106-89-0/Dihydroalprenolol; 7681-49-4/Sodium Fluoride; 86-01-1/Guanosine Triphosphate; EC 4.6.1.1/Adenylate Cyclase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 7 Year: 1981 Volume: 90 Issue: 1 First Page: 169 Last Page: 175 ID: 2111841 Publisher Id: 81239790 PubMed Id: 6265474 |
| Cell cycle changes in the adenylate cyclase of C6 glioma cells | |
Article Categories:
|
|
Previous Document: Integrity of mitochondria in a mammalian cell mutant defective in mitochondrial protein synthesis.
Next Document: Substrate adhesion of rat hepatocytes: a comparison of laminin and fibronectin as attachment protein...