Document Detail


Cell cycle changes and the ability of cells to undergo virus-induced fusion.
MedLine Citation:
PMID:  4340165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Synchronized cells of the mouse leukemic cell line, L5178Y, were examined for their ability to undergo virus-induced fusion. Fusion capacity (defined as the percentage of the original unicellular population that is induced by virus to participate in a fusion event) was measured in cells that were (i) arrested by colcemid, (ii) released from colcemid block, and (iii) released from thymidine block. The results show that fusion capacity is minimal during G1 phase (less than 10%), and rises to a maximum (69%) in arrested mitosis. Treatment of nonsynchronized cells with trypsin increased their fusion capacity to the level characteristic of cells arrested in mitosis. Colcemid-arrested cells produced twice as many hybrid clones as nonsynchronized cells after they were plated in selective medium.
Authors:
J K Stadler; E A Adelberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Jul 
Date Detail:
Created Date:  1972-09-22     Completed Date:  1972-09-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1929-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Biology*
Cell Division / drug effects
Cell Fusion / drug effects
Cell Line
Clone Cells
Cytidine / pharmacology
Demecolcine
Hybridization, Genetic
Leukemia, Experimental*
Lymphocytes* / drug effects,  growth & development
Mice
Mitosis
Parainfluenza Virus 1, Human*
Thymidine / pharmacology
Trypsin / pharmacology
Chemical
Reg. No./Substance:
477-30-5/Demecolcine; 50-89-5/Thymidine; 65-46-3/Cytidine; EC 3.4.21.4/Trypsin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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