Document Detail

Cell cycle arrest of human gingival fibroblasts and periodontal ligament cells by Actinobacillus actinomycetemcomitans: involvement of the cytolethal distending toxin.
MedLine Citation:
PMID:  15601319     Owner:  NLM     Status:  MEDLINE    
The cytolethal distending toxin (Cdt) is produced by several Gram-negative bacterial species and causes growth arrest and morphological alterations in mammalian cells. Actinobacillus actinomycetemcomitans, which is involved in the pathogenesis of localized aggressive periodontitis, also produces a Cdt that affects periodontal connective tissue cells. The aim of this study was to investigate in which phase of the cell cycle these cells are arrested and enlarged when challenged with A. actinomycetemcomitans, and to evaluate the involvement of its Cdt. Human gingival fibroblasts and periodontal ligament cells were challenged with A. actinomycetemcomitans extract, or with purified Cdt, and cell cycle analysis was performed by propidium iodide staining and flow cytometry. Cells exposed to an A. actinomycetemcomitans wild-type strain, or to purified Cdt, were arrested in both G1 and G2/M phases, and appeared enlarged compared to the corresponding controls. The cellular enlargement occurred in both G1 and G2/M arrested cells. In contrast, cells exposed to an A. actinomycetemcomitans cdt-knockout mutant strain showed cell cycle phase distribution and size similar to the controls. In conclusion, A. actinomycetemcomitans causes a combined G1 and G2/M growth arrest and enlargement in periodontal connective tissue cells, which is attributed to its Cdt.
G N Belibasakis; A Mattsson; Y Wang; C Chen; A Johansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  112     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-12-16     Completed Date:  2005-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  674-85     Citation Subset:  IM    
Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden.
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MeSH Terms
Actinobacillus actinomycetemcomitans / chemistry*,  physiology
Bacterial Toxins / pharmacology*
Cell Division / drug effects*
Cell Line
Fibroblasts / cytology,  drug effects*
Gingiva / cytology*
Periodontal Ligament / cytology*
Grant Support
Reg. No./Substance:
0/Bacterial Toxins; 0/cytolethal distending toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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