| Cell cycle analysis by cyclin E + A/DNA multiparameter flow cytometry in exponential growth MOLT-4 cells. | |
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MedLine Citation:
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PMID: 11780393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease. METHODS: Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT-4 cells, and measured by flow cytometry. RESULTS: We developed a cyclin E + A/DNA flow cytometry analysis method, which may distinguish G0, early G1, late G1, S, G2 and M phase cells, rather than three phases in the DNA content histogram. CONCLUSION: Cyclin E + A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G0, early G1, late G1, S, G2 and M phase cells. It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation. |
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Authors:
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J Qin; D Tao; D Shu; Y Leng; J Gong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chinese medical journal Volume: 114 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2002-01-08 Completed Date: 2002-02-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 967-71 Citation Subset: IM |
Affiliation:
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Surgery Department/Molecular Medical Centre, Tongji Hospital, Tongji Medical University, Wuhan 430030, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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genetics,
physiology* Cell Division / genetics, physiology Cyclin A / analysis* Cyclin E / analysis* DNA / genetics, metabolism* Flow Cytometry / methods Humans Immunohistochemistry Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Cyclin A; 0/Cyclin E; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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