Document Detail


Cell cycle analysis by cyclin E + A/DNA multiparameter flow cytometry in exponential growth MOLT-4 cells.
MedLine Citation:
PMID:  11780393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease. METHODS: Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT-4 cells, and measured by flow cytometry. RESULTS: We developed a cyclin E + A/DNA flow cytometry analysis method, which may distinguish G0, early G1, late G1, S, G2 and M phase cells, rather than three phases in the DNA content histogram. CONCLUSION: Cyclin E + A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G0, early G1, late G1, S, G2 and M phase cells. It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation.
Authors:
J Qin; D Tao; D Shu; Y Leng; J Gong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chinese medical journal     Volume:  114     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2002-01-08     Completed Date:  2002-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  967-71     Citation Subset:  IM    
Affiliation:
Surgery Department/Molecular Medical Centre, Tongji Hospital, Tongji Medical University, Wuhan 430030, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / genetics,  physiology*
Cell Division / genetics,  physiology
Cyclin A / analysis*
Cyclin E / analysis*
DNA / genetics,  metabolism*
Flow Cytometry / methods
Humans
Immunohistochemistry
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Cyclin A; 0/Cyclin E; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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