Document Detail


Cell culture studies on neurofibromatosis (von Recklinghausen). I. Comparative growth experiments with fibroblasts at high and low concentrations of fetal calf serum.
MedLine Citation:
PMID:  6793503     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth of strains of fibroblasts derived from patients with neurofibromatosis (NF) was compared with that of strains from appropriate controls in culture medium containing 1% or 15% fetal calf serum. The means of the ratios of final to initial cell numbers do not differ significantly between NF strains and control strains. Weakly significant differences are, however, obtained after conversion of the results to mean numbers of cell population doublings, the NF strains showing the higher numbers. The ratios of final to initial amounts of protein also differ significantly under both sets of growth conditions. High growth parameters occur significantly more frequently among our sample of 11 NF strains than among our sample of 13 control strains. The possibility of the expression of the NF genotype(s) on the level of the cultured fibroblast-like cells and the possible causes of the large ranges of inter- and intra-individual variations of the results are discussed.
Authors:
W Krone; S Zörlein; R Mao
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human genetics     Volume:  58     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1981  
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  188-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cell Count
Cell Division
Cells, Cultured
Culture Media
Female
Fetal Proteins
Fibroblasts / physiology
Genotype
Humans
Male
Neurofibromatosis 1 / genetics,  pathology*
Proteins / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fetal Proteins; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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