Document Detail


Cell culture platform with mechanical conditioning and nondamaging cellular detachment.
MedLine Citation:
PMID:  20358641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells implanted after injury may remodel undesirably with improper mechanical stimulation from surrounding tissue. Proper conditioning of tissue engineered constructs before implantation can lead to suitable tissue architectures, along with an extracellular matrix (ECM) environment that more closely mimics native tissue. Additionally, cell implantation without bulky polymeric scaffolding is often desirable. Previous researchers have created devices capable of applying mechanical forces to cells (e.g., stretch) but cellular removal from these devices, such as by trypsin, often results in irreversible damage. Conversely, devices are available that can detach intact cells, but these are inelastic, nonstretchable substrates. We have created a cell culture platform that allows for mechanical conditioning and then subsequent nondamaging detachment of those cells. We have modified silicone culture surfaces, to incorporate thermally responsive polymers of N-isopropylacrylamide (NIPAAm) to create an elastic substrate that can also change surface properties with temperature change. A copolymer of NIPAAm and 10percent w/w acrylic acid (AAc) was conjugated to an amine-bonded silicone surface through carbodiimide chemistry. Cells were able to attach to the resulting surfaces at 37 degreeC and showed detachment by rounded morphology at 25degreeC. Following mechanical stretching, cells were still able to spontaneously detach from these modified silicone surfaces with temperature change.
Authors:
Elaine L Lee; Horst A von Recum
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-31     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  411-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Wiley Periodicals, Inc.
Affiliation:
Department of Biomedical Engineering, Case Western Reserve University, Wickenden 220, 10900 Euclid Avenue,Cleveland, Ohio 44106, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrylamides / chemistry
Acrylates / chemistry
Animals
Biocompatible Materials / chemistry,  metabolism
Bioreactors
Cell Adhesion / physiology*
Cell Culture Techniques* / instrumentation,  methods
Cells, Cultured
Extracellular Matrix / chemistry,  metabolism
Materials Testing
Mice
Molecular Structure
NIH 3T3 Cells
Polymers / chemistry
Silicones / chemistry
Stress, Mechanical*
Surface Properties
Temperature
Tissue Engineering / instrumentation,  methods
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Acrylates; 0/Biocompatible Materials; 0/Polymers; 0/Silicones; 2210-25-5/N-isopropylacrylamide; 79-10-7/acrylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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