| Cell culture monitoring via an auto-sampler and an integrated multi-functional off-line analyzer. | |
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MedLine Citation:
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PMID: 19918877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian cell-based bioprocesses are used extensively for production of therapeutic proteins. Off-line monitoring of such cultivations via manual sampling is often labor-intensive and can introduce operator-dependent error into the process. An integrated multi-functional off-line analyzer, the BioProfile FLEX (NOVA Biomedical, Waltham MA) has been developed, which combines the functionality of three off-line analyzers (a cell counter, an osmometer, and a gas/electrolyte & nutrient/metabolite bio-profile analyzer) into one device. In addition, a novel automated sampling system has also been developed that allows the BioProfile FLEX to automatically analyze the culture conditions in as many as ten bioreactors. This is the first report on the development and function of this integrated analyzer and an auto-sampler prototype for monitoring of mammalian cell cultures. Evaluation of the BioProfile FLEX was conducted in two separate laboratories and involved two BioProfile FLEX analyzers and two sets of reference analyzers (Nova BioProfile 400, Beckman-Coulter Vi-Cell AS, and Advanced Instruments Osmometer 3900), 13 CHO cell lines and over 20 operators. In general, BioProfile FLEX measurements were equivalent to those obtained using reference analyzers, and the auto-sampler did not alter the samples it provided to the BioProfile FLEX. These results suggest that the system has the potential to dramatically reduce the manual labor involved in monitoring mammalian cell bioprocesses without altering the quality of the data obtained, and integration with a bioreactor control system will allow feedback control of parameters previously available only for off-line monitoring. |
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Authors:
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Gayle E Derfus; Daniel Abramzon; Meg Tung; David Chang; Robert Kiss; Ashraf Amanullah |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology progress Volume: 26 ISSN: 1520-6033 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2010 Jan-Feb |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-05-24 Revised Date: 2011-05-26 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 284-92 Citation Subset: IM |
Affiliation:
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Genentech Inc., Oceanside Process Research & Development, 1 Antibody Way, Oceanside, CA 92056, USA. derfus.gayle@gene.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioreactors CHO Cells Cell Culture Techniques / instrumentation*, methods* Cricetinae Cricetulus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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