Document Detail


Cell culture monitoring via an auto-sampler and an integrated multi-functional off-line analyzer.
MedLine Citation:
PMID:  19918877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian cell-based bioprocesses are used extensively for production of therapeutic proteins. Off-line monitoring of such cultivations via manual sampling is often labor-intensive and can introduce operator-dependent error into the process. An integrated multi-functional off-line analyzer, the BioProfile FLEX (NOVA Biomedical, Waltham MA) has been developed, which combines the functionality of three off-line analyzers (a cell counter, an osmometer, and a gas/electrolyte & nutrient/metabolite bio-profile analyzer) into one device. In addition, a novel automated sampling system has also been developed that allows the BioProfile FLEX to automatically analyze the culture conditions in as many as ten bioreactors. This is the first report on the development and function of this integrated analyzer and an auto-sampler prototype for monitoring of mammalian cell cultures. Evaluation of the BioProfile FLEX was conducted in two separate laboratories and involved two BioProfile FLEX analyzers and two sets of reference analyzers (Nova BioProfile 400, Beckman-Coulter Vi-Cell AS, and Advanced Instruments Osmometer 3900), 13 CHO cell lines and over 20 operators. In general, BioProfile FLEX measurements were equivalent to those obtained using reference analyzers, and the auto-sampler did not alter the samples it provided to the BioProfile FLEX. These results suggest that the system has the potential to dramatically reduce the manual labor involved in monitoring mammalian cell bioprocesses without altering the quality of the data obtained, and integration with a bioreactor control system will allow feedback control of parameters previously available only for off-line monitoring.
Authors:
Gayle E Derfus; Daniel Abramzon; Meg Tung; David Chang; Robert Kiss; Ashraf Amanullah
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  26     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-05-24     Revised Date:  2011-05-26    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  284-92     Citation Subset:  IM    
Affiliation:
Genentech Inc., Oceanside Process Research & Development, 1 Antibody Way, Oceanside, CA 92056, USA. derfus.gayle@gene.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Bioreactors
CHO Cells
Cell Culture Techniques / instrumentation*,  methods*
Cricetinae
Cricetulus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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