Document Detail


Cell culture of human colon adenomas and carcinomas.
MedLine Citation:
PMID:  3567899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell lines were established from colon adenomas, including tubular and villous polyps, primary adenocarcinomas, and metastases arising in patients with colon adenocarcinomas. The protocol for cultivating these diverse tissues includes primary cultivation of tissue explants on a type I collagen gel followed by nonenzymatic subculture of the epithelial outgrowth. All early passages were accomplished using low subculture ratios. Cultured cells elaborate morphological structures which are similar to features present in the tissues from which they were cultivated. Specifically, all structural features of colon epithelial cells were identified, including junction formation, prominent microvilli, and mucin secretion, in several cell lines. Five cell lines cultured from colonic neoplasms at different stages of cancer progression were selected for detailed characterization. Cells grown from two tubular polyps had normal human karyotypes. Cells from a villous polyp and all adenocarcinomas were aneuploid with stable marker chromosomes. The established cell lines exhibit distinct phenotypes based on growth characteristics in vitro and in athymic mice; and it is suggested that these cell lines represent useful models for studying the evolution of colon cancer from a benign to an aggressive cell type.
Authors:
J K Willson; G N Bittner; T D Oberley; L F Meisner; J L Weese
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  47     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-06-11     Completed Date:  1987-06-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2704-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics,  pathology*
Adenoma / genetics,  pathology*
Cell Line
Cells, Cultured
Collagen
Colonic Neoplasms / genetics,  pathology*
Culture Media
Culture Techniques / methods*
Humans
Karyotyping
Liver Neoplasms / secondary
Microscopy, Electron
Chemical
Reg. No./Substance:
0/Culture Media; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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