| Cell counting. | |
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MedLine Citation:
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PMID: 18770655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells. |
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Authors:
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M C Phelan; G Lawler |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Appendix 3 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Appendix 3A Citation Subset: IM |
Affiliation:
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Thompson Children's Hospital, Chattanooga, Tennessee, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count / methods* Cell Growth Processes / physiology* Cytological Techniques / instrumentation, methods* Flow Cytometry / instrumentation, methods Humans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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