Document Detail


Cell counting.
MedLine Citation:
PMID:  18770655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells.
Authors:
M C Phelan; G Lawler
Related Documents :
14731185 - Effects of tricalcium phosphate bone graft materials on primary cultures of osteoblast ...
2999165 - Separation of murine megakaryocytes and their progenitors on continuous gradients of pe...
16910935 - Application of a microfluidic device for counting of bacteria.
6287125 - Studies on thymocyte subpopulations in guinea pigs. differences in cell volume and prol...
23734815 - Fludarabine treatment favors the retention of mir-485-3p by prostate cancer cells: impl...
355235 - Chromosome replication rate and cell shape in escherichia coli: lack of coupling.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Appendix 3     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-09-04     Completed Date:  2008-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Appendix 3A     Citation Subset:  IM    
Affiliation:
Thompson Children's Hospital, Chattanooga, Tennessee, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count / methods*
Cell Growth Processes / physiology*
Cytological Techniques / instrumentation,  methods*
Flow Cytometry / instrumentation,  methods
Humans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Common stock solutions, buffers, and media.
Next Document:  Techniques for bacterial cell culture: media preparation and bacteriological tools.