Document Detail

Cell confinement in patterned nanoliter droplets in a microwell array by wiping.
MedLine Citation:
PMID:  19585570     Owner:  NLM     Status:  MEDLINE    
Cell patterning is useful for a variety of biological applications such as tissue engineering and drug discovery. In particular, the ability to localize cells within distinct fluids is beneficial for a variety of applications ranging from microencapsulation to high-throughput analysis. However, despite much progress, cell immobilization and maintenance within patterned microscale droplets remains a challenge. In particular, no method currently exists to rapidly seed cells into microwell arrays in a controllable and reliable manner. In this study, we present a simple wiping technique to localize cells within arrays of polymeric microwells. This robust method produces cell seeding densities that vary consistently with microwell geometry and cell concentration. Moreover, we develop a simple theoretical model to accurately predict cell seeding density and seeding efficiency in terms of the design parameters of the microwell array and the cell density. This short-term cell patterning approach is an enabling tool to develop new high-throughput screening technologies that utilize microwell arrays containing cells for screening applications.
Lifeng Kang; Matthew J Hancock; Mark D Brigham; Ali Khademhosseini
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  93     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-15     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  547-57     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Wiley Periodicals, Inc.
Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02115, USA.
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MeSH Terms
Cell Count
Cell Culture Techniques* / instrumentation,  methods
Cells, Cultured
Drug Discovery
Embryonic Stem Cells / cytology,  physiology
High-Throughput Screening Assays
Microarray Analysis* / instrumentation,  methods
Microfluidic Analytical Techniques* / instrumentation,  methods
Models, Theoretical
Pluripotent Stem Cells / cytology,  physiology
Polymers / chemistry
Silanes / chemistry
Grant Support
R01 EB008392-01A2/EB/NIBIB NIH HHS; R01 HL092836-02/HL/NHLBI NIH HHS; R21 EB007249-02/EB/NIBIB NIH HHS; R21 EB009196-01/EB/NIBIB NIH HHS; RL1 DE019024-02/DE/NIDCR NIH HHS; RL1 DE019024-03/DE/NIDCR NIH HHS
Reg. No./Substance:
0/Polymers; 0/Silanes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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