Document Detail


Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays.
MedLine Citation:
PMID:  19162002     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Xenopus, the animal cap is very sensitive to BMP antagonists, which result in neuralization. In chick, however, only cells at the border of the neural plate can be neuralized by BMP inhibition. Here we compare the two systems. BMP antagonists can induce neural plate border markers in both ventral Xenopus epidermis and non-neural chick epiblast. However, BMP antagonism can only neuralize ectodermal cells when the BMP-inhibited cells form a continuous trail connecting them to the neural plate or its border, suggesting that homeogenetic neuralizing factors can only travel between BMP-inhibited cells. Xenopus animal cap explants contain cells fated to contribute to the neural plate border and even to the anterior neural plate, explaining why they are so easily neuralized by BMP-inhibition. Furthermore, chick explants isolated from embryonic epiblast behave like Xenopus animal caps and express border markers. We propose that the animal cap assay in Xenopus and explant assays in the chick are unsuitable for studying instructive signals in neural induction.
Authors:
Claudia Linker; Irene De Almeida; Costis Papanayotou; Matthew Stower; Virginie Sabado; Ehsan Ghorani; Andrea Streit; Roberto Mayor; Claudio D Stern
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-01-03
Journal Detail:
Title:  Developmental biology     Volume:  327     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-02     Completed Date:  2009-04-10     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  478-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods
Biological Markers / metabolism*
Bone Morphogenetic Proteins / antagonists & inhibitors*,  genetics,  metabolism
Cell Communication / physiology*
Chick Embryo
Embryonic Induction / physiology*
GATA2 Transcription Factor / genetics,  metabolism
GATA3 Transcription Factor / genetics,  metabolism
Gene Expression Regulation, Developmental
Neural Plate / cytology,  physiology*
Transplants*
Xenopus Proteins / genetics,  metabolism
Xenopus laevis* / anatomy & histology,  embryology
Grant Support
ID/Acronym/Agency:
G0400559//Medical Research Council; GM60156/GM/NIGMS NIH HHS; R01 MH060156-01/MH/NIMH NIH HHS; R01 MH060156-02/MH/NIMH NIH HHS; R01 MH060156-03/MH/NIMH NIH HHS; R01 MH060156-04/MH/NIMH NIH HHS; R01 MH060156-05/MH/NIMH NIH HHS; R01 MH060156-06/MH/NIMH NIH HHS; //Biotechnology and Biological Sciences Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Bone Morphogenetic Proteins; 0/GATA2 Transcription Factor; 0/GATA3 Transcription Factor; 0/Xenopus Proteins
Comments/Corrections

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